Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse blood from native and Plasmodium berghei induced experimental cerebral malaria untreated or treated with activated charcoal.


ABSTRACT: We found that oral activated charcoal (oAC) provided significant protection against P. berghei ANKA-induced ECM, significantly increasing overall survival time compared to untreated mice. Protection from ECM by oAC was associated with a reduced numbers of splenic TNF+ CD4+ T cells and multifunctional IFNy+TNF+ CD4+ and CD8+ T cells. Furthermore, we identified a whole blood gene expression signature (68 genes) associated with protection from ECM. To evaluate whether oAC might affect current best available anti-malarial treatment, a group of female C57BL/6 mice infected with PbA received Activated Charcoal. At day 6 p.i., and prior to the first deaths of untreated PbA-infected mice, 5 untreated PbA-infected mice (Group 1) and 5 AC-treated PbA-infected mice (Group 2) were killed and fresh blood (300-500�l per mouse) was collected and processed into RNA. Blood was also taken from uninfected control mice (�baseline�- Group 3).

ORGANISM(S): Mus musculus

SUBMITTER: Naveed Aziz 

PROVIDER: E-MEXP-2594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Oral activated charcoal prevents experimental cerebral malaria in mice and in a randomized controlled clinical trial in man did not interfere with the pharmacokinetics of parenteral artesunate.

de Souza J Brian JB   Okomo Uduak U   Alexander Neal D ND   Aziz Naveed N   Owens Benjamin M J BM   Kaur Harparkash H   Jasseh Momodou M   Muangnoicharoen Sant S   Sumariwalla Percy F PF   Warhurst David C DC   Ward Stephen A SA   Conway David J DJ   Ulloa Luis L   Tracey Kevin J KJ   Foxwell Brian M J BM   Kaye Paul M PM   Walther Michael M  

PloS one 20100415 4


<h4>Background</h4>Safe, cheap and effective adjunct therapies preventing the development of, or reducing the mortality from, severe malaria could have considerable and rapid public health impact. Oral activated charcoal (oAC) is a safe and well tolerated treatment for acute poisoning, more recently shown to have significant immunomodulatory effects in man. In preparation for possible efficacy trials in human malaria, we sought to determine whether oAC would i) reduce mortality due to experiment  ...[more]

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