Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

44 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of Burkholderia cenocepacia antibiotic adapted resistant mutants after antibiotic stress

ABSTRACT: The response of antibiotic adapted resistant mutants of B. cenocepacia J2315 to antibiotic stress was investigated using expression profiling of three biological replicates and comparing the profiles to the J2315 parent control grown without antibiotics.
A reference design was used with Cy3 labeled genomic DNA of B. cenocepacia J2315 as common reference. Three test conditions with three biological replicates each were compared to three replicates of the control condition.
Test conditions: J2315-A grown in the presence of 250 ug per ml amikacin, J2315-M grown in the presence of 8 ug per ml meropenem and J2315-T grown in the presence of 60 ug per ml trimethoprim and 300 ug per ml sulfamethoxazole.
Control condition: J2315 parent strain grown without antibiotics.

ORGANISM(S): Burkholderia cenocepacia

SUBMITTER: Andrea Sass

PROVIDER: E-MEXP-2708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling by array of Burkholderia cenocepacia J2315 wild type and antibiotic-resistant mutants exposed to sub-inhibitory concentrations of drugs

Project description:Burkholderia cenocepacia J2315 wild type and adaptive mutants with elevated resistance to antibiotics were exposed to sub-inhibitory concentrations of drugs

2010-08-01 | E-MEXP-2738 | biostudies-arrayexpress

Changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis

Project description:Global gene expression in three clinical isolates of the ET12 lineage of Burkholderia cenocepacia was compared by microarray analysis: Strain J2315 (isolated 1989), and strains BCC1616 (HI4277) and BCC1617 (HI4283), both isolated in 2008.

2010-08-02 | E-MEXP-2747 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown to mid-log phase in different media

Project description:B. cenocepacia J2315 was grown to mid-log phase in different media: LB broth, iso-sensitest broth, basal salt medium with glucose at pH 7 and pH 6, basal salt medium at pH 7 with a reduced iron content, and basal salt medium with glycerol

2012-01-01 | E-MEXP-2762 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 from the beginning of the stationary phase compared to mid-log phase

Project description:Burkholderia cenocepacia J2315 was grown in LB broth to the beginning of stationary phase. The expression profile was compared to cultures harvested in mid-log phase.

2012-01-01 | E-MEXP-2754 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown at 20C or 37C

Project description:B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at O.D. 0.5 at 150 rpm in a shaking incubator at two different temperatures: 37 degrees and 20 degrees centigrade.

2012-01-01 | E-MEXP-2789 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 at the beginning of the stationary phase compared to mid-log phase

Project description:Burkholderia cenocepacia J2315 was grown at 37 degrees centigrade in LB broth to the beginning of stationary phase (18 hours incubation time). The expression profile was compared to cultures harvested in mid-log phase (7 hours incubation time).

2012-01-01 | E-MEXP-2790 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown to mid-stationary phase in basal salt medium with glucose or glycerol substrates

Project description:B. cenocepacia J2315 was grown to mid-stationary phase in basal salt medium with two different substrates: 20 mM glucose or 40 mM glycerol. Cells were harvested after 30 hours incubation at 37 degrees centigrade. The expression profile was compared to cells grown on the same medium and harvested in mid-log phase.

2012-01-01 | E-MEXP-2772 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown under low or atmospheric oxygen concentration conditions

Project description:B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at OD 0.3 under full aeration and then transferred into a 50 ml centrifuge tube and placed upright into a CampyGen Compact (Oxoid, Basingstoke, UK) plastic pouch containing the gas generating paper sachet. The pouch was sealed immediately and then the cap of the centrifuge tube loosened to allow exchange of atmosphere between centrifuge tube and pouch. The culture was further incubated at 37 degrees centigrade and 150 rpm for 2-3 hours until it reached OD 0.5. The centrifuge tube was sealed before opening the pouch and then snap-cooled before harvest to minimise exposure to atmospheric oxygen and to ensure expression profiles reflect the lower oxygen concentration. The expression profile was compared to cells grown to OD 0.5 at atmospheric oxygen concentrations.

2012-01-01 | E-MEXP-2787 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species

Project description:B. cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species. To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested. To expose cultures to oxidative stress by reactive oxygen species, cells were grown at 37ºC to an OD of 0.5. Then t butyl hydroperoxide or hydrogen peroxide solution were added to the culture at 0.001% and 0.15% final concentration. The culture was further incubated for 15 min and then harvested. The expression profiles were compared to cells grown in LB medium without exposure to stress.

2012-01-01 | E-MEXP-2791 | biostudies-arrayexpress

Transcription profiling of Burkholderia cenocepacia J2315 sessile cells to treatment with high doses of hydrogen peroxide, sodium hypochlorite and chlorhexidine

Project description:In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown Burkholderia cenocepacia cells against high concentrations of disinfectants, the present study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells following exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.

2010-03-02 | E-MEXP-2502 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data