Dataset Information
Transcription profiling by array of Burkholderia cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species
Ontology highlight
ABSTRACT:
To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested.
To expose cultures to oxidative stress by reactive oxygen species, cells were grown at 37ºC to an OD of 0.5. Then t butyl hydroperoxide or hydrogen peroxide solution were added to the culture at 0.001% and 0.15% final concentration. The culture was further incubated for 15 min and then harvested.
The expression profiles were compared to cells grown in LB medium without exposure to stress.
ORGANISM(S): Burkholderia cenocepacia
SUBMITTER: Andrea Sass
PROVIDER: E-MEXP-2791 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia.
The ISME journal 20130314 8
Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. B. cenocepacia is known for its ability to cause lung infections in people with cystic fibrosis and it possesses a large 8 Mb multireplicon genome encoding a wide array of pathogenicity and fitness genes. Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made w ...[more]
