Project description:Comparison of RNA levels between wild type and a Kdo Transferase mutant in Arabidopsis thaliana. We are interested by the cell-wall biosyntheis in the Arabidopsis thaliana plants which are affected in Kdo biosynthesis.
Project description:Identification of molecular target of MGO3 genes. 2 first leaves of mgo3-2 mutant or wild-type plants (WS ecotype) were harvested from plants grown during 2 weeks in vitro on MS medium in long days conditions. 3 repeats have been done for each samples
Project description:1- Comparison of the transcriptome of two ecotypes (Col0 and Ler0): compare the transcriptome of ColO versus Ler0 for different tissues and developmental stages (seedlings 7 days, shoot 14 days, flowers, leafs 35 days)<br> 2- Impact of SINE RNA : Analyse the consequence of the expression of a SINE RNA in Arabidopsis thaliana<br> 3- Impact of the gcn2 mutation : Impact of the gcn2 mutation on the Arabidopsis thaliana transcriptome. GCN2 is a kinase potentially regulated by the SINE non-coding RNA
Project description:T-DNA insertions in the HAF2 gene are characterised. The mutation of the gene induced production of pale young leaves. Genetic analysis showed that HAF2 is required for both phytochrome and cryptochrome signalling. Accordingly, light-inducible gene expression and CAB2 promoter activity were affected by the mutation. The present transcriptome analysis showed that the mutation affected about 9% of Arabidopsis genes in 14 day-old leaves.
Project description:Comparison between wild type Wassilewskija (WS) Arabidopsis plants and HCA mutant 10 days after planting. 3 biological repetitions have been done.
Project description:Arabidopsis cells (5 days after subculturing) were cultured at 21°C, 8 h photoperiod under agitation. H2O2 2.5 µM was added to cells 5 days after subculturing and 2 hours after the beginning of the light period. Treated and control cells were collected 15 min, 1 h, 5 h and 12 h after treatment.
Project description:Technical replicates hybridisations using 1.04 Arabidopsis thaliana - columbia-0 plants :plants cultivated and RNA-extracted by VIB, amplified and hybridised at URGV. aVIB-Col_vs_aVIB-Col
Project description:hybridisations using 1.04 Arabidopsis thaliana - columbia-0 and landsberg plants:plants cultivated and RNA-extracted by VIB, amplified and hybridised at URGV. aVIB-Ler_vs_aVIB-Col
Project description:hybridisations using 1.04 Arabidopsis thaliana - columbia-0 plants :plants cultivated and RNA-extracted by VIB, amplified and hybridised at URGV (VIB-Col)and plants cultivated and RNA-extracted by URGV, amplified and hybridised at URGV (URGV-Col). aVIB-Col_vs_aURGV-Col