Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide transcriptional profiling of Bacillus subtilis greA deletion and GreA-D44A mutant strains


ABSTRACT: Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that E. coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Furthermore, Gre factors participate in stimulation of promoter escape and suppression of promoter-proximal pausing during beginning of RNA synthesis in E. coli. Although Gre factors are conserved in general bacteria, limited functional studies have been performed in bacteria other than E. coli. In this investigation, ChAP-chip analysis was conducted to visualize the distribution of B. subtilis GreA on the chromosome and determine the effects of GreA inactivation on core RNAP trafficking. Our data show that GreA inactivation induces RNAP accumulation at many promoter or promoter-proximal regions. Additionally, we performed transcriptome comparison between in wild type and greA deletion and greA D44A mutant cells to see the effect of RNAP pausing to the transcriptomes. Our results indicate that inactivation of GreA has a limited impact on the transcriptome, and these effects are not directly related to RNAP accumulation in the promoter or promoter-proximal regions.

ORGANISM(S): Bacillus subtilis

SUBMITTER: Taku Oshima 

PROVIDER: E-MEXP-3055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcription factor GreA contributes to resolving promoter-proximal pausing of RNA polymerase in Bacillus subtilis cells.

Kusuya Yoko Y   Kurokawa Ken K   Ishikawa Shu S   Ogasawara Naotake N   Oshima Taku T  

Journal of bacteriology 20110422 12


Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Furthermore, Gre factors participate in the stimulation of promoter escape and the suppression of promoter-proximal pausing during the beginning of RNA synthesis in E. coli. Although G  ...[more]

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