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Regulation of initiation of chromosome replication by oriC-proximal DnaA-box clusters in Bacillus subtilis

ABSTRACT: Comparison of transcriptions in wild_type, delta-6 (deletion of six DnaA-box clusters (DBCs)), delta-5 and delta-DBC[yydA-yycS] strains, to see effect of the DBCs on transcription of neighboring genes and DnaA-regulated genes.

ORGANISM(S): Bacillus subtilis

SUBMITTER: Shu Ishikawa

PROVIDER: E-MEXP-3069 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of chromosomal replication initiation by oriC-proximal DnaA-box clusters in Bacillus subtilis.

Okumura Hajime H Yoshimura Mika M Ueki Mikako M Oshima Taku T Ogasawara Naotake N Ishikawa Shu S

Nucleic acids research 20110912 1

Bacterial chromosome replication is initiated by binding of DnaA to a DnaA-box cluster (DBC) within the replication origin (oriC). In Bacillus subtilis, six additional DBCs are found outside of oriC and some are known to be involved in transcriptional regulation of neighboring genes. A deletion mutant lacking the six DBCs (Δ6) initiated replication early. Further, inactivation of spo0J in Δ6 cells yielded a pleiotropic phenotype, accompanied by severe growth inhibition. However, a spontaneous su ...[more]

PMID: 21911367

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