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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA post transcriptional control of umbilical cord mesenchymal stem cell osteogenic differentiation.

ABSTRACT: Umbilical cord mesenchymal stem cells were stimulated to osteogenesis by 24h, 48h and 7 days. Undifferentiated and treated cells have their total RNA extracted and 25 ug were used to extract micro RNAs by Flash PAGE Fractionator (Ambion). These miRNA were labeled and used to hybridize to 662 oligo arrays corresponding to miRNA sequences from human, rat and mouse. The hybridized slides were scanned in the Amersham Automatic Slide Processor, quantified by TIGR Spotfinder software and submitted to stathiscal treatment in TIGR MEV software.

ORGANISM(S): Homo sapiens

SUBMITTER: Geraldo Passos

PROVIDER: E-MEXP-3080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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