Project description:Sporophytes of Saccharina latissima were exposed for two weeks to 12 different combinations of photosynthetically active radiation and UVR at three different temperatures (2,7,12 C). Maximum quantum yield of photosystem II was determined twice a week during the experimental duration for observing the extent of photoinhibition. For investigating molecular mechanisms of acclimation to high photosynthetically radiation, and UVR gene expression profiles were assessed through microarray hybridizations.

2012-04-01 | E-MEXP-3556 | biostudies-arrayexpress

Light and temperature induced gene expression in Saccharina latissima

Project description:Effect of PAR and temperature stress on the gene expression Saccharina latissima. Total RNA of stress treatments (low PAR 2° and 17°C, high PAR 2° and 17°C) was hybridized against the control treatment (low PAR 12°C); hybridizations were carried out in 4 replicates.

2012-01-07 | E-MEXP-3450 | biostudies-arrayexpress

Transcription profiling by array of Sordaria macrospora wild type strain undergoing sexual development and the mutant strains dSmtA-1 and dSmtA-2

Project description:Cross-species hybridizations of Sordaria macrospora targets on Neurospora crassa microarrays were performed with targets derived from the S. macrospora wild type undergoing sexual development (wt.sex) and the mutant strains dSmtA-1 and dSmtA-2. For each strain, two independent experiments were carried out with a dye switch in the second experiment. The following slide/target/dye combinations were done: slide N16-86: Cy3-target: wt.sex (1), Cy5-target: dSmtA-1 (1) slide N16-87: Cy3-target: dSmtA-1 (2), Cy5-target: dSmtA-2(2) slide N16-88: Cy3-target: dSmtA-2 (1), Cy5-target: wt.sex (2)

2010-04-29 | E-MEXP-2600 | biostudies-arrayexpress

Chromatin immunoprecipitation to measure RNA polymerase II occupancy in fission yeast

Project description:The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays

2007-03-22 | E-TABM-219 | biostudies-arrayexpress

Transcription profiling by array of Antarctic krill across the moult cycle relative to an early intermoult stage

Project description:To generate differential gene expression profiles across the moult cycle of Antarctic krill, relative to an early intermoult stage.

2011-03-03 | E-MEXP-2605 | biostudies-arrayexpress

Alexandrium_CO2

Project description:Effect of different pCO2 levels on Alexandrium tamarense

2013-08-15 | E-MEXP-3946 | biostudies-arrayexpress