Project description:Exposing freshly isolated human articular chondrocytes to hyperosmotic conditions (550 mOsm vs 380mOsm controls) for 5 hours and then performing transcriptome analysis using Illumina Ref8 arrays.
Project description:Transgenic Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07-1.08) under a 16 h light/8 h dark regimen (40 ± 10 ?mol photons/m2/s) at 22 C
Project description:Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07-1.08) under a 16 h light/8 h dark regimen (40 ± 10 ?mol photons/m2/s) at 22 C. The light intensity was the same at 22 C and at 4 C. Plants were harvested 2 h later after lights were turned on. Cold treatment: The 3-week-old plants were transferred from 22 C to 4 C and were grown for 1 or 4 days.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 M-1 10 ?mol photons/m2/s) at 28M-0C (day) and 25M-0C (night). For cold treatment, two-week-old plants were transferred from 28M-0C to 10M-0C and incubated for 1 day.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 ± 10 ?mol photons/m2/s) at 28°C (day) and 25°C (night). For dehydration treatment, two-week-old plants were incubated for 3 days without watering. The soil moisture content was 15.6% on day 3 of dehydration.
Project description:IGR39 cells, seeded at a density of 6x104 cells/well in 12-well culture plates, were transfected with 5 nM of miR-211 mimic or negative control (NCM) as described in the main text. Samples for total RNA extraction were collected 48h after transfection. RNA quality was assessed using RNA 6000 NanoChips with the Agilent 2100 Bioanalyzer (Agilent, Paolo Alto, USA). Gene expression profiling experiments using GeneChipM-. Human Gene 2.0 ST arrays and miRNA profiling using GeneChipM-. miRNA arrays (Affymetrix, Santa Clara, CA, USA) were performed on two independent samples at the CRP-SantM-i Microarray Unit (Luxembourg) as described before (Reinsbach et al., 2012) and according to standard protocols. Standard pipeline from the PartekM-. Genomics SuiteTM (Partek GS) software was used for analysis of data files. Lists of genes were generated by pair-wise comparison of expression data sets (negative control vs. mimic treated samples at 48h).
Project description:Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07-1.08) under a 16 h light/8 h dark regimen (40 ± 10 ?mol photons/m2/s) at 22 C.Dehydration treatment: The 3-week-old plants were grown for 2 or 3 days without watering. To obtain accurate results, we carefully raised single plants in Petri dishes, each containing an equal amount of soil. Soil moisture contents were calculated from soil dry weight. Untreated; the soil moisture content was 84.3%. Under dehydration, on the second day, the soil moisture content was 51.1%. Under dehydration, on the third day, the moisture content was 11.6%.
Project description:Here we studied the leaf transcriptome in maize,<br>throughout a cycle of 10 h darkness and 14 h light to look for rhythmic patterns<br>of gene expression.
Project description:NCI-H1618 cells treated on d0 and d3 with 25 nM control or ASCL1-targeting siRNA. Biologic triplicate RNA samples harvested at day 5.