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Transcriptional Profile of Perinatal Rat Pancreas

ABSTRACT: Pancreata were excised from offspring of time-mated Wistar rats age 10-11 weeks (Taconic, Lille Skensved, Denmark) at embryonic day 20 (E20), immediately after birth (P0) and two days after birth (P2). After decapitation, individual pancreata were quickly dissected and placed directly in ice cold TRIReagent (Sigma-Aldrich, St Louis, MO, USA). RNA from individual pancreata was kept separately in order to verify the array results in pools of RNA as well as in individual animals by real time qPCR. 0.5 M-5g of RNA from each of 3 male and 3 female littermates were pooled at each time-point (E20, P0 and P2). This was repeated in each of three biological independent replicate experiments giving a total of 9 pooled samples, three at each time points

ORGANISM(S): Rattus norvegicus

SUBMITTER: Louise Gaarn

PROVIDER: E-MEXP-3174 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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