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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expansion of HSC by AML1a

ABSTRACT: Mouse bone marrow cells were infected with retrovirus for doxycylin-controllable AML1a expression, and cultured in vitro. Cells were treated with doxycycline to silence AML1a expression or left untreated to keep AML1a expression on, and subjected to microarray analysis to profile transcripts AML1a influences.

ORGANISM(S): Mus musculus

SUBMITTER: Shinobu Tsuzuki

PROVIDER: E-MEXP-3179 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expansion of functionally defined mouse hematopoietic stem and progenitor cells by a short isoform of RUNX1/AML1.

Tsuzuki Shinobu S Seto Masao M

Blood 20111130 3

Self-renewal activity is essential for the maintenance and regeneration of the hematopoietic system. The search for molecules capable of promoting self-renewal and expanding hematopoietic stem cells (HSCs) has met with limited success. Here, we show that a short isoform (AML1a) of RUNX1/AML1 has such activities. Enforced AML1a expression expanded functionally defined HSCs, with an efficiency that was at least 20 times greater than that of the control in vivo and by 18-fold within 7 days ex vivo. ...[more]

PMID: 22130803

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