Project description:The objectif was to investigated the anti-atherogenic effects of nutritional supplementation of NAR in different mouse models of hypercholesterolemia and use transcriptomic approach to identify its molecular targets in the liver and aorta.
Project description:At eight weeks of age, male mice were divided into two groups (15 mice per group) fed iso-energetic diet: a control-diet or the same diet supplemented with 0.2% of curcumin (Sigma Aldrich) for 16 weeks (Table S1). The dose of curcumin used in this study is equivalent to 1g curcumin consumption in humans, a level of consumption achievable in South-Asian countries. No significant difference in weight gain was observed between the two groups at the end of the experimental period (data not shown). After 16 weeks, mice were anaesthetized (40 mg pentobarbital/kg of body weight) and blood was collected from the abdominal aorta into heparinised tubes and plasma was stored at -80 C. After washing with sterilized PBS, aorta and heart samples were immediately frozen in liquid nitrogen and stored at -80 C. Original processed data are available in the archive: http://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3185/E-MEXP-3185.additional.zip
Project description:We have previously showed a strong genetic determinant governing resistance of the M. truncatula A17 line to C. trifolii is located in a chromosomal region at the top of chromosome 4 (Ameline-torregrosa et al., 2008). This region also contains the RCT1 gene which has been shown to confer resistance to C. trifolii when transferred to a susceptible alfalfa plants (Zhu et al.), In order to evaluate the role of this region in the response to non-adapted Colletorichum species and to compare this response to those induce by C. trifolii, two M. truncatula near-isogenic lines differing only in this chromosomal region were used for transcript profiling experiments. These two lines were issued from a recombinant-inbred collection obtained from a A17-F83005.5 cross (Cazaux et al., in preparation). Plant inoculations were done on two-week-old entire plants by spraying a conidial suspension of C. trifolii or C. lindemuthianum to avoid possible artifacts which can be observed on detached leaves (Liu et al., 2007). RNA was extracted from leaves collected at 1dpi and 3dpi and transcript profiling was performed using Mt16kOLI1Plus chips (Thompson et al., 2005).
Project description:Here we studied the leaf transcriptome in maize,<br>throughout a cycle of 10 h darkness and 14 h light to look for rhythmic patterns<br>of gene expression.