FOXO3a and beta-catenin transcriptional profile in human colon cancer cell line DLD1
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ABSTRACT: We established a model system in human DLD1 colon cancer cells to study the transcriptional crosstalk between FOXO3A and beta-Catenin. Thereby, translocation to the nucleus of a AKT-insensitive mutant (T32A, S253A, S315A) of human FOXO3A fused to the ligand binding domain of human estrogen receptor can be induced by exposure to 4-hydroxy-tamoxifen. Furthermore, expression of a stable mutant (S33Y) of human beta-catenin is doxycycline inducible. Addition of those drugs separately or in combination allows identification of common or excusive target gene sets.
ORGANISM(S): Homo sapiens
SUBMITTER: Stephan Tenbaum
PROVIDER: E-MEXP-3262 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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