Dataset Information

Human platelet β-catenin interaction proteome

ABSTRACT: In nucleated cells, β-catenin, the key downstream effector of this pathway, is a dual function protein, regulating the coordination of gene transcription and cell–cell adhesion. The specific role of β-catenin in the anucleate platelet however remains elusive. Here, we performed a label-free quantitative proteomic analysis of β-catenin immunoprecipitates from human platelets identifying 9 co-immunoprecipitating proteins. GO biological pathway analysis revealed a significant enrichment of specific functional terms including 'cell adhesion', 'cell junction organization' and ‘adherens junction organization'. Our bioinformatics data suggests that human platelet β-catenin may be involved in facilitating cell adhesion and cell junctions. We found three proteins co-immunoprecipitating with β-catenin under both resting and activated conditions, four proteins under resting condition only and two proteins under activated condition only.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Platelet, Platelet

SUBMITTER: Paulina Szklanna

LAB HEAD: Patricia B. Maguire

PROVIDER: PXD008130 | Pride | 2018-07-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	140214kw_TIM_1_1_1Resting_IP_Donor1_Rep1.raw	Raw
	140214kw_TIM_1_1_2Resting_IP_Donor1_Rep2.raw	Raw
	140214kw_TIM_1_1_3Resting_IP_Donor1_Rep3.raw	Raw
	140214kw_TIM_1_2_1Resting_IgG_Donor1_Rep1.raw	Raw
	140214kw_TIM_1_2_2Resting_IgG_Donor1_Rep2.raw	Raw

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Publications

Proteomic Analysis Reveals a Strong Association of β-Catenin With Cadherin Adherens Junctions in Resting Human Platelets.

Maguire Patricia B PB Donlon Tim T Parsons Martin M Wynne Kieran K Dillon Eugene E Ní Áinle Fionnuala F Szklanna Paulina B PB

Proteomics 20180501 9

It was previously demonstrated that the WNT/β-catenin pathway is present and active in platelets and established that the canonical WNT ligand, WNT-3a, suppresses platelet adhesion and activation. In nucleated cells, β-catenin, the key downstream effector of this pathway, is a dual function protein, regulating the coordination of gene transcription and cell-cell adhesion. The specific role of β-catenin in the anucleate platelet however remains elusive. Here, a label-free quantitative proteomic a ...[more]

PMID: 29510447

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