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Shifting of immune responsiveness to house dust mite by influenza a infection genomic insights

ABSTRACT: House dust mite (HDM) extract (Greer Laboratories, Lenoir, NC) was resuspended in sterile phosphate-buffered saline (PBS) at a concentration of 0.5 mg (protein)/ml and 10 ul (5 ug dose) was administered to isofluorane-anesthetized mice intranasally. Groups of mice were infected either with influenza A or exposed to PBS. Seven days later, separate groups of mice were either exposed to saline or HDM for 3 or 6 days and lungs harvested 24 h after the last exposure, at day 4 (early phase; EP) or day 7 (late phase; LP).

ORGANISM(S): Mus musculus

SUBMITTER: Mainul Husain

PROVIDER: E-MEXP-3325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Shifting of immune responsiveness to house dust mite by influenza A infection: genomic insights.

Al-Garawi Amal A Husain Mainul M Ilieva Dora D Humbles Alison A AA Kolbeck Roland R Stampfli Martin R MR O'Byrne Paul M PM Coyle Anthony J AJ Jordana Manel M

Journal of immunology (Baltimore, Md. : 1950) 20111214 2

Respiratory viral infections have been associated with an increased incidence of allergic asthma. However, the mechanisms by which respiratory infections facilitate allergic airway disease are incompletely understood. We previously showed that exposure to a low dose of house dust mite (HDM) resulted in enhanced HDM-mediated allergic airway inflammation, and, importantly, marked airway hyperreactivity only when allergen exposure occurred during an acute influenza A infection. In this study, we ev ...[more]

PMID: 22174454

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