Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression in M82-GAME1-RNAi plants vs Wild type

ABSTRACT: We introduced the GAME1i construct into the indeterminate M82 cultivar by crossing, obtaining plants that displayed a phenotype of severe growth retardation, deformed leaves and abortion of flower buds . In addition, leaves of these plants exhibited dark necrotic spots resembling symptoms typically seen after infection of tomato by pathogenic bacteria like Xanthamonas campestris pv. vesicatoria (Xcv) or Pseudomonas syringae pv. tomato (Pst) . As no other tomato lines grown in the same greenhouse at the same time showed similar disease-like symptoms, we hypothesized that silencing GAME1 might mimic induction of disease symptoms in the absence of a pathogen. Indeed, we were able to isolate neither Xcv nor Pst (or other putative pathogens) from GAME1i leaves that displayed necrotic spots. Microarray analysis was performed to examine if the transgene, and possibly, the corresponding changes in the metabolic profile, induced the plant response system at the transcriptional level.

ORGANISM(S): Lycopersicon esculentum

SUBMITTER: Maxim Itkin

PROVIDER: E-MEXP-3455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

GLYCOALKALOID METABOLISM1 is required for steroidal alkaloid glycosylation and prevention of phytotoxicity in tomato.

Itkin Maxim M Rogachev Ilana I Alkan Noam N Rosenberg Tally T Malitsky Sergey S Masini Laura L Meir Sagit S Iijima Yoko Y Aoki Koh K de Vos Ric R Prusky Dov D Burdman Saul S Beekwilder Jules J Aharoni Asaph A

The Plant cell 20111216 12

Steroidal alkaloids (SAs) are triterpene-derived specialized metabolites found in members of the Solanaceae family that provide plants with a chemical barrier against a broad range of pathogens. Their biosynthesis involves the action of glycosyltransferases to form steroidal glycoalkaloids (SGAs). To elucidate the metabolism of SGAs in the Solanaceae family, we examined the tomato (Solanum lycopersicum) GLYCOALKALOID METABOLISM1 (GAME1) gene. Our findings imply that GAME1 is a galactosyltransfer ...[more]

PMID: 22180624

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Dataset Information

Gene expression in M82-GAME1-RNAi plants vs Wild type

Publications

GLYCOALKALOID METABOLISM1 is required for steroidal alkaloid glycosylation and prevention of phytotoxicity in tomato.

Similar Datasets

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