Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Transcription profiling of Sinorhizobium meliloti genes upon rpoE1 over-expression


ABSTRACT: S. meliloti genes under the control of RpoE1 were identified by determining the whole genome
transcription profile of a strain over-expressing rpoE1 under the control of the arabinose-dependent
PBAD promoter of plasmid pMLBAD-rpoE1. A strain containing the empty vector pMLBAD
was used as a reference.

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: Claude Bruand 

PROVIDER: E-MEXP-3471 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Sinorhizobium meliloti sigma factors RpoE1 and RpoE4 are activated in stationary phase in response to sulfite.

Bastiat Bénédicte B   Sauviac Laurent L   Picheraux Carole C   Rossignol Michel M   Bruand Claude C  

PloS one 20121130 11


Rhizobia are soil bacteria able to establish a nitrogen-fixing symbiosis with legume plants. Both in soil and in planta, rhizobia spend non-growing periods resembling the stationary phase of in vitro-cultured bacteria. The primary objective of this work was to better characterize gene regulation in this biologically relevant growth stage in Sinorhizobium meliloti. By a tap-tag/mass spectrometry approach, we identified five sigma factors co-purifying with the RNA polymerase in stationary phase: t  ...[more]

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