Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

ER in liver

ABSTRACT: In order to elucidate the mechanism underlying ER control of liver metabolic function, we investigated the genetic programs controlled by the hepatic receptor during the physiological fluctuation of circulating E2 in adult female mice. To this aim, we used Affymetrix GeneChip Arrays and compared the expression of hepatic genes in mice in two phases of the estrous cycle: Proestrus (P) and Metestrus (M), characterized by high and low circulating E2 respectively. Analysis of data demonstrated that the expression of genes involved in lipid and cholesterol metabolism changes during the reproductive cycle. To further elucidate ER transcriptional activity at M and P, we performed a whole genome ChIP experiment followed by tiling array. We observed that at M, but not at P, recruited ERs are mostly in proximity (20 kbp) of genes involved in energy metabolism, thus strengthening the hypothesis of ER playing a bridging role between metabolism and reproduction.

ORGANISM(S): Mus musculus

SUBMITTER: Alessandro Villa 

PROVIDER: E-MEXP-3504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Tetradian oscillation of estrogen receptor α is necessary to prevent liver lipid deposition.

Villa Alessandro A   Della Torre Sara S   Stell Alessia A   Cook Jennifer J   Brown Myles M   Maggi Adriana A  

Proceedings of the National Academy of Sciences of the United States of America 20120703 29

In the liver of female mice, the transcriptional activity of estrogen receptor (ER) α oscillates in phase with the 4-d-long estrous cycle. Here systemic, genome-wide analysis demonstrates that ER tetradian oscillation is necessary to generate pulses of expression in genes for fatty acid and cholesterol synthesis. This ER-dependent metabolic programming changes with pregnancy and after cessation of ovarian function due to age or surgical menopause, suggesting that ER signaling is optimized to coo  ...[more]

Similar Datasets

Transcriptomics ER in Liver - 2
2012-07-04 | E-MEXP-3505 | biostudies-arrayexpress
Transcriptomics The TMAO Generating Enzyme Flavin Monooxygenase 3 is a Central Regulator of Cholesterol Balance
2014-12-19 | E-GEOD-64326 | biostudies-arrayexpress
Proteomics Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in the liver of male mouse
2015-12-23 | PXD002025 | Pride
Genomics Genome-wide analysis of Estrogen Receptor-mediated response to acute Estradiol treatment in liver (ChIP-seq)
2016-05-13 | E-GEOD-70346 | biostudies-arrayexpress
Proteomics mouse liver microsomes phosphorylation
2015-08-24 | PXD002221 | Pride
Proteomics mouse liver microsomes phosphorylation with LC-MS/MS
2017-04-04 | PXD003043 | Pride
Transcriptomics Transcription profiling of rodent hepatic carcinogenesis
2004-03-08 | E-MEXP-89 | biostudies-arrayexpress
Proteomics Proteomic analysis of liver proteome affected by intrauterine growth restriction in newborn piglets.
2016-03-15 | PXD003524 | Pride
Transcriptomics The TMAO Generating Enzyme Flavin Monooxygenase 3 is a Central Regulator of Cholesterol Balance
2014-12-19 | GSE64326 | GEO
Transcriptomics Genome-wide analysis of Estrogen Receptor-mediated response to acute Estradiol treatment in liver (expression)
2016-05-13 | E-GEOD-70329 | biostudies-arrayexpress