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Gene expression profiling of the effect of elevated TRIB3 expression on the transcriptional response to glucose deprivation

ABSTRACT: Gene expression profiling of HEK293-derived cell lines that are stably transfected with either a tetracycline-inducible TRIB3 expression construct (TRIB3-293 cells), tetracycline-inducible ATF4 expression construct (ATF4-293 cells) or the corresponding empty vector (Vector-293 cells). Samples from tetracycline-treated and tetracycline-untreated cell cultures were collected after a 24-hour (TRIB3-293 cells, Vector-293) or 4-hour (ATF4-293) incubation in growth medium either with or without glucose.

ORGANISM(S): Homo sapiens

SUBMITTER: Tiit Örd

PROVIDER: E-MTAB-3318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TRIB3 enhances cell viability during glucose deprivation in HEK293-derived cells by upregulating IGFBP2, a novel nutrient deficiency survival factor.

Örd Tiit T Örd Daima D Adler Priit P Vilo Jaak J Örd Tõnis T

Biochimica et biophysica acta 20150618 10 Pt A

Glucose deprivation occurs in several human diseases, including infarctions and solid tumors, and leads to cell death. In this article, we investigate the role of the pseudokinase Tribbles homolog 3 (TRIB3) in the cellular stress response to glucose starvation using cell lines derived from HEK293, which is highly glycolytic under standard conditions. Our results show that TRIB3 mRNA and protein levels are strongly upregulated in glucose-deprived cells via the induction of activating transcriptio ...[more]

PMID: 26094770

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