Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AsrR regulon

ABSTRACT: Enterococcus faecium is an important opportunistic pathogen emerging in hospitals worldwide. We identified a new MarR family global regulator in E. faecium, named AsrR (antibiotic and stress response regulator). We phenotipically characterized key role for AsrR in E. faecium pathogenicity. The aim of the microarray-based experiments was to investigate the AsrR regulon in E. faecium. We constructed a mutant strain deleted for the asrR gene, we complemented the mutant and, finally, we observed genes expression in these strains in comparison with the wild-type strain. (The parental HM1070 was used to delete the asrR gene and to obtain the mutant strain. Then, the mutant strain was used to restore the asrR gene and to obtain the complemented strain.) This approach will allow us to identify the genes regulated by AsR to clarify its role in E. faecium.

ORGANISM(S): Enterococcus faecium

SUBMITTER: Francois Lebreton

PROVIDER: E-MEXP-3528 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome wt vs mdxR mutant in E. faecium E1162 grown in BHI

Project description:Comparative transcriptome analysis of E. faecium E1162 and its isogenic mdxR deletion mutant during growth in BHI in mid-exponential phase (A660 = 0.3)

2013-10-09 | E-MEXP-3760 | biostudies-arrayexpress

Transcriptome wt vs mdxR mutant in E. faecium E1162 grown in M1 with maltose

Project description:Comparative transcriptome analysis of E. faecium E1162 and its isogenic mdxR deletion mutant during growth in M1 with maltose in mid-exponential phase (A660 = 0.3)

2013-10-09 | E-MEXP-3759 | biostudies-arrayexpress

Reproducibility M-Tram of Enterococcus faecium strain E1162

Project description:Two independent aliquots containing approximately 10^7 cells from the transposon mutant of E. faecium E1162 were grown at 37 C for 20 hours in 20 ml of BHI broth. Genomic DNA was isolated from the two replicate cultures and used for the generation of cDNA. The cDNA samples were labeled with Cy3 and Cy5 respectively and hybridized to a microarray that was designed using the E. faecium E1162 genome sequence.

2012-11-02 | E-MEXP-3502 | biostudies-arrayexpress

M-TraM E.faecium BHI and BHI with bile salts

Project description:A genome-wide identification of the genetic loci required for bile salts resistance in E. faecium

2013-10-10 | E-MEXP-3797 | biostudies-arrayexpress

FP-biotin enrichment of serine hydrolases in Enterococcus faecium

Project description:In our study, we employed activity-based protein profiling (ABPP), a technique that uses specialized inhibitors to identify active serine hydrolases in different strains of E. faecium (clade A1 and A2) and E. lactis under various growth conditions. Serine hydrolases, a large and diverse family of enzymes that include established drug targets like penicillin-binding proteins, have other less-studied subfamilies. In addition to fluorescent, gel-based profiling, we used a biotin-tagged fluorophosphonate probe for the enrichment and identification of serine hydrolase enzymes via streptavidin enrichment and liquid chromatography/mass spectrometry analysis. This led to the discovery of 11 largely unexplored potential targets (including α,β-hydrolases, SGNH-hydrolases, phospholipases, amidases, and peptidases) that could be exploited for drug developmentagainst the vancomycin-resistant E. faecium strain E745.

2024-06-22 | PXD048798 | Pride

Tiling path analysis of Listeria monocytogenes wild type and prfA, sigB and hfq deletion mutants grown in different media and at different temperatures

Project description:Comparison of Listeria monocytogenes transcripts in different growth conditions (exponential phase 37 degrees C, stationary phase 37 degrees C, exponential phase 30 degrees C, hypoxia, human blood, murine intestinal lumen) and different mutant strains (wild-type versus prfA, sigB and hfq deletion mutants).

2009-04-24 | E-MEXP-2142 | biostudies-arrayexpress

Transcription profiling of Listeria monocytogenes wild type and prfA, sigB and hfq deletion mutants grown in different media and at different temperatures

Project description:Comparison of Listeria monocyotgenes gene expression in different growth conditions (exponential phase 37 degrees C, stationary phase 37 degrees C, exponential phase 30 degrees C, hypoxia, human blood, murine intestinal lumen) and different mutant strains (wild-type versus prfA, sigB and hfq deletion mutants).

2010-04-16 | E-MEXP-2138 | biostudies-arrayexpress

Microarray-based transposon mapping analysis of Enterococcus faecium E1162 that lost biofilm formation ability in a biofilm semi-static model

Project description:Mapping of transposon mutant library in Enterococcus faecium during growth in Brain Heart Infusion (BHI) broth and in a semi-static biofilm model. The goal of this study was to identify factors that play a role in E. faecium biofilm formation by selection of transposon insertion mutants that lost the capacity to form biofilm in vitro.

2016-05-01 | E-MTAB-4524 | biostudies-arrayexpress

Transcriptome analysis Enterococcus faecium during mid-exponential growth phase in presence and absence of ampicillin

Project description:Effect of 20 ug/ml ampicillin on gene expression of Enterococcus faecium E1162 during mid-exponential growth phase

2012-03-01 | E-MEXP-3564 | biostudies-arrayexpress

Enterococcus faecium E1162 transcriptome 25 C vs 37 C

Project description:The transcriptome of Enterococcus faecium E1162 growing in Brain heart Infusion Broth was compared in the mid-exponential growth phase (A660 = 0.3) at 25 C and 37 C.

2013-09-01 | E-MEXP-3941 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data