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Unknown,Transcriptomics,Genomics,Proteomics

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AsrR regulon

ABSTRACT: Enterococcus faecium is an important opportunistic pathogen emerging in hospitals worldwide. We identified a new MarR family global regulator in E. faecium, named AsrR (antibiotic and stress response regulator). We phenotipically characterized key role for AsrR in E. faecium pathogenicity. The aim of the microarray-based experiments was to investigate the AsrR regulon in E. faecium. We constructed a mutant strain deleted for the asrR gene, we complemented the mutant and, finally, we observed genes expression in these strains in comparison with the wild-type strain. (The parental HM1070 was used to delete the asrR gene and to obtain the mutant strain. Then, the mutant strain was used to restore the asrR gene and to obtain the complemented strain.) This approach will allow us to identify the genes regulated by AsR to clarify its role in E. faecium.

ORGANISM(S): Enterococcus faecium

SUBMITTER: Francois Lebreton

PROVIDER: E-MEXP-3528 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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