Project description:Comparison of transcript profiles of E. coli LZ41 and LZ54 fishns mutant strains containing drug-resistant alleles of different topoisomerase genes to distinguish gene transcripts associated either with relaxation or hypernegative supercoiling in absence of transcriptional regulator FIS and H-NS.
Project description:Comparison of transcript profiles of E. coli LZ41fishns and LZ54fishns strains containing drug-resistant alleles of different topoisomerase genes to distinguish gene transcripts associated either with relaxation or hypernegative supercoiling.
Project description:Identification of a stable gene expression signature with high classifying potential to discriminate benign (Follicular adenomas) and malignant (papillary carcinomas) thyroid tumors
Project description:Arabidopsis rosette leaves were harvested from plants grown under <br><br>different photoperiods under 100 M-5mol photons m-2 s-1 at 20 M-0C. <br><br><br><br>In the first experiment plants grown under short day conditions 8L/16D (8 h light / 16 h dark) for 4 weeks were compared with plants <br><br>grown under long day (16L/8D) for 3 weeks.<br><br><br><br>In the second experiment plants grown under 12L/12D <br><br>for 2 weeks were compared with plants grown first 2 weeks under <br><br>12L/12D and then two days under short day (8L/16D) conditions.
Project description:Acute myeloid leukemia (AML) is the most common and severe acute leukemia in adults. It is a heterogeneous disease where the subset of molecularly different types, presenting various morphological features and differentiation stage, can be distinguished. Genomic research of leukemias is conducted since 1999 and large cohort studies shown that particular genetic alterations correspond with specific gene expression signatures. However, not always they provide clinically relevant information. The most unknown group is cytogenetically normal acute myeloid leukemia (CN-AML, 40-49% of all AML cases). The aim of our experiment was to determine selected gene expression profiles in CN-AML, using small, boutique microarray. The array contained 933 oligonucleotide probes, mainly complementary to acute myeloid leukemia markers, genes involved in leukemic transformation and myeloid cell proliferation, differentiation and maturation. Our test dataset included 40 hybridizations: 24 corresponding with blood and bone marrow samples collected from 12 patients with AML M1 or M2 FAB subtype and 16 corresponding with healthy control samples. Total RNA was extracted from the mononuclear cell fractions, reversibly transcribed to cDNA and labeled with Alexa 647 dye. The common reference was RNA isolated from HL-60 cell culture, labeled with Alexa 555 dye.
Project description:Correlation analysis of the expression of bud dormancy-related genes in 10 peach cultivars, with different chilling requirements for dormancy release.
Project description:In previous studies, we identified the fungal macrocyclic lactone (S)-curvularin (SC) as an anti-inflammatory agent using a screening system detecting inhibitors of the Janus kinase/signal transducer and activator of transcription pathway. The objective of the present study was to investigate whether SC is able to decrease proinflammatory gene expression in an in vivo model of a chronic inflammatory disease. Therefore, the effects of SC and dexamethasone were compared in the model of collagen-induced arthritis (CIA) in mice. In addition to measuring the arthritis index (paw swelling) of the animals, we also performed whole genome microarray analyses to identify SC target genes and new therapeutic targets of SC.
Project description:Comparison of gene expression profiles between pediatric patients with and without symptoms of cross-allergy (birch-apple syndrome).