Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

47 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

OncomiR-21 triggers growth arrest of endothelial cells via a complex network involving p21 CDK2 CDC25A and NFIB

ABSTRACT: miRNA transcription in senescent human umbilical vein endothelial cells (HUVEC) compared to young HUVECs.

ORGANISM(S): Homo sapiens

SUBMITTER: Matthias Hackl

PROVIDER: E-MEXP-3596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human fibroblasts from foreskin to determine changes in miRNA regulation with age

Project description:Foreskin samples were derived from young and old donors. Total RNA was extracted and used for hybridization to microRNA microarrays. From 4 young and 4 old donors, differential expression of microRNAs was analyzed using R/Bioconductor and linear models

2010-04-19 | E-MEXP-2459 | biostudies-arrayexpress

MicroRNA profiling of human dermal fibroblasts - miR-17 and miR-19b to investigate down-regulatation in human aging

Project description:Normal human fibroblasts were isolated from skin biopsies of two different healthy causcasian patients, a 41-years old female (HDF-1) and a 16-years old male (HDF-5). HDF-5 and HDF-1 cells were cultivated in DMEM/HAMs F-12 medium (Biochrom KG, Berlin, Germany) supplemented with 10% fetal calf serum (FCS) 4 mM L-glutamine (Sigma) and Primocin antibiotics (100 µg/mL) grown at 37 °C in ambient atmosphere containing 5% CO2. Cells were grown until confluence was reached and routinely passaged using 1:4 or 1:3 split ratios. Upon decrease in growth rate and entry of irreversible growth arrest split ratio was changed to 1:2 until cells stopped dividing and fresh medium was added at least every 7 days. HDF-1 cells reached senescence after 54 population doublings, while HDF-5 cells had stopped growing after 72.7 population doublings (see Fig. S2 for growth curves and staining of senescent associated beta-galactosidase).<br>Senescent HDF RNA samples were compared to young HDF RNA samples in terms of microRNA expression using Exiqon LNA microRNA microarrays.

2010-04-19 | E-MEXP-2386 | biostudies-arrayexpress

MicroRNA profiling of human umbical vein endothelial cells (HUVEC) using young (replicating) cells and senescent cells

Project description:From two donors of human umbilical vein endothelial cells, in vitro cell lines were established. Both cell lines were grown in vitro until irreversible growth arrest was observed (replicative senescence). Total RNA from young (replicating) cells as well as senescent cells was harvested and used for hybridization of microRNA chips (MRC) from TU Graz based on Sanger miRBase 9.2

2010-04-19 | E-MEXP-2425 | biostudies-arrayexpress

MicroRNA profiling of human renal tubular epithelial cells - miR-17 and miR-19b to investigate their down-regulatation in human aging

Project description:Renal Tubular Epithelial Cells (RPTEC) from two donors were cultivated in vitro until irreversible growth arrest was observed. Total RNA from young (replicating) as well as growth arrested cells was harvested and hybridized to LNA based microRNA microarrays using a looped design. After data processing, differential expression of miRNAs in senescent vs. young RPTEC cells was calculated.

2010-04-19 | E-MEXP-2393 | biostudies-arrayexpress

MicroRNA profiling of human CD8 T cells - miR-17 and miR-19b to investigate down-regulatation in human aging

Project description:T cells from several blood donors were separated in populations corresponding to CD8+ CD28+ and CD8+ CD28- surface markers. Total RNA extracts from cells were hybridized to MRC LNA microarray. Contrasts of interest were CD8CD28- minus CD8CD28+ (replicative aging) and CD8CD28+ (old donors) minus CD8CD28+ (young donors), which corresponds to chronological aging.

2010-04-19 | E-MEXP-2398 | biostudies-arrayexpress

Transcription profiling of human miRNA expression in mesenchymal stem cells in young and old donors.

Project description:Mesenchymal stem cells were isolated from several donors of different age (young vs old), and total RNA was extracted. Samples were labeled with Cy3 and Cy5 dyes and hybridized in a looped design that allowed the calculation of differentially expressed miRNAs in MSCs isolated from healthy, aged individuals.

2010-04-19 | E-MEXP-2455 | biostudies-arrayexpress

Upregulation of miR-24 is associated with decreased DNA damage repair in highly differentiated CD8-positive T cells

Project description:microRNA transcript levels in CD8+ T-cells were profiled. Two experimental factors were considered: CD28 status (positive/negative) and Etoposide (10 M-5g/ml) treatment (etoposide/control).

2012-07-12 | E-MEXP-3307 | biostudies-arrayexpress

miRNA profile of five hematopoietic cell lines

Project description:We characterized the global microRNA expression of five commonly used hematopoietic cell lines: Kasumi-1, REH, U-937, BV-173 and ME-1. RNA samples from all five cell lines were compared to reference material composed of RNA from 20 different tissues

2014-09-01 | E-MEXP-3944 | biostudies-arrayexpress

MicroRNA profiling by array of mouse bleomycin induced lung fibrosis

Project description:mouse lung fibrosis model was established. Total RNA from mouse lungs was isolated. miRNA array analysis was performed.

2010-06-30 | E-MEXP-2749 | biostudies-arrayexpress

MicroRNA profiling of human peripheral blood leukocytes before and after stimulation with E. coli lipopolysaccharide

Project description:To gain insight into miRNAs involved in the regulation of the human innate immune response, we screened for differentially expressed miRNAs in circulating leukocytes in an in vivo model of acute inflammation triggered by E. coli lipopolysaccharide (LPS) infusion.<br>Leukocyte RNA was isolated from venous blood samples obtained from healthy male volunteers before and 4 hours after LPS-infusion. After fluorescence labeling, RNA samples were hybridized to microarrays containing capture probes for measuring the abundance<br>of more than 600 human miRNAs (Exiqon).

2008-12-01 | E-MEXP-1860 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data