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MicroRNA profiling of human peripheral blood leukocytes before and after stimulation with E. coli lipopolysaccharide

ABSTRACT: To gain insight into miRNAs involved in the regulation of the human innate immune response, we screened for differentially expressed miRNAs in circulating leukocytes in an in vivo model of acute inflammation triggered by E. coli lipopolysaccharide (LPS) infusion.
Leukocyte RNA was isolated from venous blood samples obtained from healthy male volunteers before and 4 hours after LPS-infusion. After fluorescence labeling, RNA samples were hybridized to microarrays containing capture probes for measuring the abundance
of more than 600 human miRNAs (Exiqon).

ORGANISM(S): Homo sapiens

SUBMITTER: Wolfgang Schmidt

PROVIDER: E-MEXP-1860 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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