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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MiR-99a let7c cluster

ABSTRACT: To investigate the function of miR99a/let-7c miRNAs during cardiomyogenesis, we decided to perturb their expression using transgenic mES cell lines the corresponding precursors (pre-miRNA) of the two miRNAs. We used the ROSA-TET system (EB3 tet off), in which the transgene is inserted in Rosa26 locus by Cre recombinase. We generated three different clones both miRNAs let-7c/miR-99a and those only one miRNA, namely the let-7cdeletedmiR-99a and the miR-99adeletedlet-7c.

ORGANISM(S): Mus musculus

SUBMITTER: Gilda Cobellis

PROVIDER: E-MEXP-3748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cardiomyogenesis is controlled by the miR-99a/let-7c cluster and epigenetic modifications.

Coppola Antonietta A Romito Antonio A Borel Christelle C Gehrig Corinne C Gagnebin Maryline M Falconnet Emilie E Izzo Antonella A Altucci Lucia L Banfi Sandro S Antonarakis Stylianos E SE Minchiotti Gabriella G Cobellis Gilda G

Stem cell research 20131128 2

Understanding the molecular basis of cardiomyocyte development is critical for understanding the pathogenesis of pre- and post-natal cardiac disease. MicroRNAs (miRNAs) are post-transcriptional modulators of gene expression that play an important role in many developmental processes. Here, we show that the miR-99a/let-7c cluster, mapping on human chromosome 21, is involved in the control of cardiomyogenesis by altering epigenetic factors. By perturbing miRNA expression in mouse embryonic stem ce ...[more]

PMID: 24365598

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