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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Pulse labeling and fractionation of RNA after inhibition of miR-277 to detect transcriptional and post-transcriptional changes

ABSTRACT: Drosophila melanogaster Schneider cells (S2) were transfected with a 2'-O-methyl inhibitor antisense to miR-277 or an unrelated control sequence. Three days after inhibition of the miRNA, the cellular RNA was pulse-labeled with 4-thio-Uridine for 1 hour, then tthe cells were lysed and RNA was extracted with Trizol. Following extraction, the RNA was further fractionated by in vitro biotinylation of incorporated 4-thio-uridine, yielding altogether three RNA fractions for each sample: total, newly transcribed (=labeled) and > 1h old (=unlabeled).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Klaus Foerstemann

PROVIDER: E-MEXP-3784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Drosophila miR-277 controls branched-chain amino acid catabolism and affects lifespan.

Esslinger Stephanie Maria SM Schwalb Björn B Helfer Stephanie S Michalik Katharina Maria KM Witte Heidi H Maier Kerstin C KC Martin Dietmar D Michalke Bernhard B Tresch Achim A Cramer Patrick P Förstemann Klaus K

RNA biology 20130430 6

Development, growth and adult survival are coordinated with available metabolic resources, ascertaining that the organism responds appropriately to environmental conditions. MicroRNAs are short (21-23 nt) regulatory RNAs that confer specificity on the RNA-induced silencing complex (RISC) to inhibit a given set of mRNA targets. We profiled changes in miRNA expression during adult life in Drosophila melanogaster and determined that miR-277 is downregulated during adult life. Molecular analysis rev ...[more]

PMID: 23669073

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