Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

M-TraM E.faecium BHI and BHI with bile salts

ABSTRACT: A genome-wide identification of the genetic loci required for bile salts resistance in E. faecium

ORGANISM(S): Enterococcus faecium

SUBMITTER: Xinglin Zhang

PROVIDER: E-MEXP-3797 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Functional genomic analysis of bile salt resistance in Enterococcus faecium.

Zhang Xinglin X Bierschenk Damien D Top Janetta J Anastasiou Iacovos I Bonten Marc J M MJ Willems Rob J L RJ van Schaik Willem W

BMC genomics 20130503

<h4>Background</h4>Enterococcus faecium is a Gram-positive commensal bacterium of the mammalian intestinal tract. In the last two decades it has also emerged as a multi-resistant nosocomial pathogen. In order to survive in and colonize the human intestinal tract E. faecium must resist the deleterious actions of bile. The molecular mechanisms exploited by this bacterium to tolerate bile are as yet unexplored.<h4>Results</h4>In this study we used a high-throughput quantitative screening approach o ...[more]

PMID: 23641968

Similar Datasets

M-TraM Enterococcus faecium E1162 BHI and BHI with ampicillin

Project description:Mapping of transposon mutant library during growth in Brain Heart Infusion (BHI) broth and BHI broth with 20 ug/ml ampicillin

2012-10-09 | E-MEXP-3501 | biostudies-arrayexpress

Reproducibility M-Tram of Enterococcus faecium strain E1162

Project description:Two independent aliquots containing approximately 10^7 cells from the transposon mutant of E. faecium E1162 were grown at 37 C for 20 hours in 20 ml of BHI broth. Genomic DNA was isolated from the two replicate cultures and used for the generation of cDNA. The cDNA samples were labeled with Cy3 and Cy5 respectively and hybridized to a microarray that was designed using the E. faecium E1162 genome sequence.

2012-11-02 | E-MEXP-3502 | biostudies-arrayexpress

Transcriptome wt vs mdxR mutant in E. faecium E1162 grown in BHI

Project description:Comparative transcriptome analysis of E. faecium E1162 and its isogenic mdxR deletion mutant during growth in BHI in mid-exponential phase (A660 = 0.3)

2013-10-09 | E-MEXP-3760 | biostudies-arrayexpress

M-TraM analysis for identification of chlorhexidine resistance genes in Enterococcus faecium E1162

Project description:Microarray-based Transposon Mapping (M-TraM) of E. faecium E1162 to identify conditionally essential genes, required for growth in the presence of chlorhexidine (1.2 ug/ml)

2016-07-03 | E-MTAB-4173 | biostudies-arrayexpress

Transcriptome of E.faecium E1162 under bile salts activation

Project description:This experiment is to investigate E.faecium E1162 transcriptome profiles under bile salts activation.

2013-10-10 | E-MEXP-3796 | biostudies-arrayexpress

Transcriptome wt vs mdxR mutant in E. faecium E1162 grown in M1 with maltose

Project description:Comparative transcriptome analysis of E. faecium E1162 and its isogenic mdxR deletion mutant during growth in M1 with maltose in mid-exponential phase (A660 = 0.3)

2013-10-09 | E-MEXP-3759 | biostudies-arrayexpress

Gene expression analysis of breast adenocarninoma cell line T47D treated with particulate matter PM2.5 sampled near the municipal solid waste incineration plant of Bologna city.

Project description:We reported the gene expression profile of T47D cells treated with the organic extract of Particulate matter 2.5 (PM2.5) sampled next to the municipal solid waste incineration plant of Bologna city. Based on a air pollution distribution model that takes the incinaration plant as point source of emission, two sites were chosen to sample particulate matter near incineration plant: "FrulloEst" representing the maximum effect of the incineration plant, "Calamosco" representing the negative control of "FrulloEst" (minimun effect of incineration plant, same effect of other air pollution fonts). Another site, "Giardini Margherita", is chosen to sample the urban background air pollution. for each site sample collection was performed in winter and in summer season.

2018-01-31 | E-MEXP-3686 | biostudies-arrayexpress

Sensitivity-resistance to the atypical retinoid ST1926 in NB4 acute myeloid leukemia cells

Project description:The two cell lines NB4 and NB4.437r, respectively sensitive and resistant to the cytotoxic action of the atypical retinoid ST1926 and CD437 were used to unveil the mechanism of action of these drugs.We compared their transcriptional profiles in conditions of exponential growth in basal medium by microarray gene expressio analysis.

2013-01-08 | E-MEXP-3752 | biostudies-arrayexpress

Comparative genomic hybridization of disease causing strains of Streptococcus dysgalactiae ssp.

Project description:Group C Streptococcus dysgalactiae subsp. dysgalactiae (GCS) field strains causative of bovine mastitis and group C or group G Streptococcus dysgalactiae subsp. equisimilis (GCS/GGS) isolates from human infections were tested for the presence of virulence genes employing a microarray containing 220 virulence genes of S. pyogenes (GAS).

2011-05-05 | E-MEXP-3168 | biostudies-arrayexpress

A key role for Mbd3 in 5-hydroxymethylcytosine-dependent gene regulation in embryonic stem cells

Project description:Numerous chromatin regulators are required for embryonic stem (ES) cell self-renewal and pluripotency, but few have been studied in detail. Here, we examine the roles of several chromatin regulators whose loss affects the pluripotent state of ES cells. We find that Mbd3 and Brg1 antagonistically regulate a common set of genes by regulating promoter-proximal nucleosome occupancy and recruitment of RNA polymerase II. Furthermore, both Mbd3 and Brg1 play key roles in the biology of 5-hydroxymethylcytosine (5hmC): Mbd3 colocalizes with both Tet1 and 5hmC in vivo, its localization is Tet1-dependent, and binding of Mbd3/NURD to DNA in vitro is inhibited by methylcytosine, but not hydroxymethylcytosine. Finally, both Mbd3 and Brg1 are themselves required for normal levels of 5hmC in vivo. Together, our results identify an effector for 5hmC, and reveal that control of gene expression by antagonistic chromatin regulators is a surprisingly common regulatory strategy in ES cells. Genomic binding profiles of Mbd3 in normal ES cells, along with ES cells depleted of additional chromatin regulators, Brg1 and Tet1 were performed by ChIP-seq. In addition, we performed mapping of RNA Polymerase II in control (EGFP), Mbd3 and Brg1 knockdown ES cells by ChIP-seq.

2011-12-01 | E-GEOD-31690 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data