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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gamma-Catenin is expressed throughout normal human hematopoietic development and is required for normal PU.1-dependent monocyte differentiation

ABSTRACT: CD34+ HPC cells were retrovirally transduced with either control PINCO-GFP or PINCO Gamma-Catenin-GFP and analysed for Affymetrix mRNA expression. Gamma-Catenin and control matched transduced cells were also cultured until day 6 and were fractionated into the main progenitor groups for Affymetrix microarray analysis as previously described (tonks 2007). Briefly, a depletion strategy using the Miltenyi Biotec MiniMACS system was used for this purpose where, myeloblasts (CD15+) were enriched from mixed lineage transduced cells by serial depletion of monoblasts (CD14hi) and erythroblasts (CD36+) using immunomagnetic beads directed against those antigens.

ORGANISM(S): Homo sapiens

SUBMITTER: Alex Tonks

PROVIDER: E-MEXP-3810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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