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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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NIH3T3 transfected with myocardin

ABSTRACT: NIH3T3 cell transiently transfected with myocardin-EGFP or EGFP. Transfection: Lipofectamine 2000 according to the manufacturers instructions (Invitrogen).

ORGANISM(S): Mus musculus

SUBMITTER: Thomas Boettger

PROVIDER: E-MEXP-3869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-1/133a clusters cooperatively specify the cardiomyogenic lineage by adjustment of myocardin levels during embryonic heart development.

Wystub Katharina K Besser Johannes J Bachmann Angela A Boettger Thomas T Braun Thomas T

PLoS genetics 20130919 9

miRNAs are small RNAs directing many developmental processes by posttranscriptional regulation of protein-coding genes. We uncovered a new role for miR-1-1/133a-2 and miR-1-2/133a-1 clusters in the specification of embryonic cardiomyocytes allowing transition from an immature state characterized by expression of smooth muscle (SM) genes to a more mature fetal phenotype. Concomitant knockout of miR-1-1/133a-2 and miR-1-2/133a-1 released suppression of the transcriptional co-activator myocardin, a ...[more]

PMID: 24068960

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