Project description:Endothelial cells derived from freshly purified human islets were incubated with microvesicles collected by ultracentrifugation of supernatants of endothelial progenitor cells (EPCs) isolated from peripheral blood of healthy volunteers.
Project description:proximal tubular epithelial cell apoptosis were incubated with 5% plasma of septic patients or healthy volunteers diluited in RPMI 1640
Project description:TECs cultured in normoxic conditions, TECs cultured in hypoxic conditions and TECs cultured in hypoxic treated with EPc derived microvesicles
Project description:Human pancreatic islets were isolated from pancreas of deceased donors by Ricordi's procedure and cultured in CMRL 1066 medium additioned with human albumin. EVs were isolated from conditioned medium derived from islet culture after isolation. Once isolated, RNA of islets and islet-derived EVs was extracted and analyzed for microRNA expression within 48 hours after isolation.
Project description:Identification of transcriptional profile of several genes involved in diabetes in islet-derived extracellular vesicles (Evs). Recently, EVs are identified as a new mechanism in cell-to-cell communication by transfer of protein and genic information (mRNA, microRNA). Their role is under investigation in immunology, stem cell and cancer, but not in islets and diabetes. The aim of this experiment is to identify mRNA transcripts (in particular, mRNA transcripts involved in diabetes pathophysiology) present in islet Evs.
Project description:Tubular endothelial cells were cultured in hypoxic conditions (1% O2) and treated or not with microvesicles derived from endothelial progenitor cells.<br>mRNA profiling of hypoxic cells, treated or not with MVs, was analyzed after nromalization with mRNA profiling of normoxic cells.<br><br>This experiment was updated on 27th Jan 2011 to correct descriptions.
Project description:In order to investigate the molecular mechanisms activated by deleterious BRCA1 variants in yeast, the RNA obtained from yeast cells transformed with five variants exhibiting a phenotypic effect, either on proliferation and/or on HR was hybridized on microarrays in comparison with the RNA from cells transformed with wild-type BRCA1