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Deciphering the role of RND efflux transporters in Burkholderia cenocepacia D4

ABSTRACT: To compare the expression profile of B. cenocepacia mutants impaired in RND efflux pump encoding genes

ORGANISM(S): Burkholderia cenocepacia

SUBMITTER: Silvia Bazzini

PROVIDER: E-MEXP-2999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deciphering the role of RND efflux transporters in Burkholderia cenocepacia.

Bazzini Silvia S Udine Claudia C Sass Andrea A Pasca Maria Rosalia MR Longo Francesca F Emiliani Giovanni G Fondi Marco M Perrin Elena E Decorosi Francesca F Viti Carlo C Giovannetti Luciana L Leoni Livia L Fani Renato R Riccardi Giovanna G Mahenthiralingam Eshwar E Buroni Silvia S

PloS one 20110419 4

Burkholderia cenocepacia J2315 is representative of a highly problematic group of cystic fibrosis (CF) pathogens. Eradication of B. cenocepacia is very difficult with the antimicrobial therapy being ineffective due to its high resistance to clinically relevant antimicrobial agents and disinfectants. RND (Resistance-Nodulation-Cell Division) efflux pumps are known to be among the mediators of multidrug resistance in gram-negative bacteria. Since the significance of the 16 RND efflux systems prese ...[more]

PMID: 21526150

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Transcription profiling by array of Burkholderia cenocepacia J2315 wild type and antibiotic-resistant mutants exposed to sub-inhibitory concentrations of drugs

Project description:Burkholderia cenocepacia J2315 wild type and adaptive mutants with elevated resistance to antibiotics were exposed to sub-inhibitory concentrations of drugs

2010-08-01 | E-MEXP-2738 | biostudies-arrayexpress

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Project description:The response of antibiotic adapted resistant mutants of B. cenocepacia J2315 to antibiotic stress was investigated using expression profiling of three biological replicates and comparing the profiles to the J2315 parent control grown without antibiotics. A reference design was used with Cy3 labeled genomic DNA of B. cenocepacia J2315 as common reference. Three test conditions with three biological replicates each were compared to three replicates of the control condition. Test conditions: J2315-A grown in the presence of 250 ug per ml amikacin, J2315-M grown in the presence of 8 ug per ml meropenem and J2315-T grown in the presence of 60 ug per ml trimethoprim and 300 ug per ml sulfamethoxazole. Control condition: J2315 parent strain grown without antibiotics.

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