Project description:imbibed Col-0 seeds were sown on ? X MS medium supplemented with 0.8% agar and 1% sucrose. The plates were first exposed to continuous light for 12 h to stimulate germination and then wrapped with two layers of aluminum foil and placed in the growth chamber for 5 d. Once the plant material was uniformly germinated, the experimental conditions were applied. 5-d-old, dark-grown seedlings were washed seven times with sterile water followed by a wash with ? X MS liquid medium without sucrose to remove residual exogenous sugar and the plant material was kept in ? X MS liquid without sucrose in the dark for all subsequent steps. Cultures were shaken at 140 rpm at 22 C for 24 h and then treated with ? X MS without glucose or ½ X MS supplemented with BR (100 nM), glucose (3%), or glucose (3%) + BR (100 nM) for 3 h. Seedlings were harvested after 3h and preceded for RNA isolation and microarray analysis.
Project description:Analysis of the effect of heterologous expression of a soybean DREB2 gene (GmDREB2A;2)on the transcriptome of transgenic Arabidopsis plants. For the genetic modification of Arabidopsis, we introduced gene casettes that express our genes of interest into the genome viaT-DNA transfer using Agrobacterium tumefaciens.
Project description:Wild-type A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07M-^V1.08) under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22C. Cold treatment: The 3-week-old plants were transferred from 22C to 4C and were grown for 1 day.