Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of degreening transgenic Arabidopsis over time

ABSTRACT: Transgenic Arabidopsis thaliana bearing a synthetic degreening circuit were induced to degrade their chlorophyll, and the transcriptomes were profiled at 0, 24, and 48h after degreening induction, as well as in 48h plants that were subsequently allowed to "regreen". Induction of transgene expression resulted in overexpression of the chlorophyll degrading enzymes chorophyllase and PAO (pheide a oxygenase), as well as expression of a diRNA construct to the chlorophyll biosynthetic POR enzymes (NADPH:protochlorophyllide oxidoreductase).

INSTRUMENT(S): arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: June Medford 

PROVIDER: E-MEXP-505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.

Antunes Mauricio S MS   Ha Suk-Bong SB   Tewari-Singh Neera N   Morey Kevin J KJ   Trofka Anna M AM   Kugrens Paul P   Deyholos Michael M   Medford June I JI  

Plant biotechnology journal 20061101 6

Plants have evolved elegant mechanisms to continuously sense and respond to their environment, suggesting that these properties can be adapted to make inexpensive and widely used biological monitors, or sentinels, for human threats. For a plant to be a sentinel, a reporting system is needed for large areas and widespread monitoring. The reporter or readout mechanism must be easily detectable, allow remote monitoring and provide a re-set capacity; all current gene reporting technologies fall shor  ...[more]

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