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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of flax (Linum usitatissimum) developmental series

ABSTRACT: Three 2cm segments were excised from different parts (TOP, MID, BOT) along the vertical axis of a 4 week old (25cm) stem of flax (L. usitatissimum) were compared using a cDNA amplicon array. Each segment represented a different developmental stage, especially in relation to bast fibre differentiation (i.e. TOP= elongation, MID=transition, BOT= thickening). Only the cDNAs that showed the highest differential expression were sequenced.

INSTRUMENT(S): arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Linum usitatissimum

SUBMITTER: Michael Deyholos

PROVIDER: E-MEXP-990 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis of flax (Linum usitatissimum L.) stems identifies transcripts enriched in fibre-bearing phloem tissues.

Roach Melissa J MJ Deyholos Michael K MK

Molecular genetics and genomics : MGG 20070515 2

To better understand the molecular processes associated with the development of the unusually long (> 30 mm) and strong bast fibre cells within the phloem of flax stems, we conducted a gene discovery experiment to identify transcripts enriched in fibre-bearing tissues, with the intention that these transcripts would serve as future targets for crop improvement and research in phloem development and cell wall deposition. We produced a library of 9,600 cDNA clones from the peels of flax stems, and ...[more]

PMID: 17503083

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