Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

59 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of flax (Linum usitatissimum) developmental series

ABSTRACT: Three 2cm segments were excised from different parts (TOP, MID, BOT) along the vertical axis of a 4 week old (25cm) stem of flax (L. usitatissimum) were compared using a cDNA amplicon array. Each segment represented a different developmental stage, especially in relation to bast fibre differentiation (i.e. TOP= elongation, MID=transition, BOT= thickening). Only the cDNAs that showed the highest differential expression were sequenced.

INSTRUMENT(S): arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Linum usitatissimum

SUBMITTER: Michael Deyholos

PROVIDER: E-MEXP-990 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Microarray analysis of flax (Linum usitatissimum L.) stems identifies transcripts enriched in fibre-bearing phloem tissues.

Roach Melissa J MJ Deyholos Michael K MK

Molecular genetics and genomics : MGG 20070515 2

To better understand the molecular processes associated with the development of the unusually long (> 30 mm) and strong bast fibre cells within the phloem of flax stems, we conducted a gene discovery experiment to identify transcripts enriched in fibre-bearing tissues, with the intention that these transcripts would serve as future targets for crop improvement and research in phloem development and cell wall deposition. We produced a library of 9,600 cDNA clones from the peels of flax stems, and ...[more]

PMID: 17503083

Similar Datasets

Transcription profiling of three developmental stages of the outer layer of hemp (Cannabis sativa) stems

Project description:Three 2cm segments were excised from different parts (TOP, MID, BOT) along the vertical axis of a 4 week old stem of hemp (C. sativa), and the outer layers of the stem were compared using a cDNA amplicon array. Each segment represented a different developmental stage, especially in relation to bast fibre differentiation (i.e. TOP= elongation, MID=transition, BOT= thickening). Only the cDNAs that showed the highest differential expression were sequenced.

2007-07-01 | E-MEXP-1042 | biostudies-arrayexpress

Transcription profiling of flax stems and leaves from 4 week old flax plants to identify transcripts enriched in stem and phloem bast fibres

Project description:Stems and leaves from 4 week old flax plants were hybridized to an array of cDNAs from the outer (i.e. phloem enriched) layer of stems of the same age to identify transcripts enriched in stem (and phloem bast fibres).

2007-05-01 | E-MEXP-989 | biostudies-arrayexpress

Transcription profiling of degreening transgenic Arabidopsis over time

Project description:Transgenic Arabidopsis thaliana bearing a synthetic degreening circuit were induced to degrade their chlorophyll, and the transcriptomes were profiled at 0, 24, and 48h after degreening induction, as well as in 48h plants that were subsequently allowed to "regreen". Induction of transgene expression resulted in overexpression of the chlorophyll degrading enzymes chorophyllase and PAO (pheide a oxygenase), as well as expression of a diRNA construct to the chlorophyll biosynthetic POR enzymes (NADPH:protochlorophyllide oxidoreductase).

2006-04-01 | E-MEXP-505 | biostudies-arrayexpress

Transcription profiling of left and right lateral plates of mouse 8.5dpc embryos to identify assymetrically expressed genes

Project description:C3H/HeH females were mated with 101/H males Mating was assessed by the presence of vaginal plugs the next morning. Identification of plug was designated day 0.5. At 8.5 days of development the dam was culled by cervical dislocation, the uterine horns disscted and placed into PBS. The uterus was dissected to release decuduae and these opened according to standard protocols. Morphologically normal looking embryos of between 4 and 6 somites were collected. The left and right lateral plates were dissected away with watchmakers forceps and pools of 4 left and 4 right lateral plates were snap frozen in LN2.

2010-06-29 | E-MEXP-2277 | biostudies-arrayexpress

Transcription profiling of Baseline gene expression in circulating peripheral blood mononuclear cells isolated from 18 apparently healthy volunteers (10 females and 8 males)

Project description:Baseline gene expression in circulating peripheral blood mononuclear cells isolated from 18 apparently healthy volunteers (10 females and 8 males). The volunteers fasted overnight for 10 hours prior to collection of blood samples (but they were allowed water). 5 blood samples were collected from each volunteer at consecutive 8-day intervals. Volunteers were included in this study provided they met the following selection criteria, that they should be ?Aged between 20 and 45 years of age; ?A non-smoker (or an ex-smoker); ?Not pregnant or been pregnant in the last 12 months ?Not diagnosed with any long-term medical condition; ?Or have not (in the previous 4 months) donated blood, received any inoculations and are not taking regular prescribed medicine (including HRT and oral contraception) Prior to inclusion in this study volunteers were screened for general health and had a blood sample taken for routine screening of Hb content etc. Only those volunteers who were in good general health were allowed to participate in the study. The age of all volunteers has been reported here as being 20-45 years in order to preserve the anonymity of the data, and so that the individuals involved may not be able to track down their own specific data. Please contact us if the details of volunteers' ages are required.

2013-01-30 | E-TABM-7 | biostudies-arrayexpress

Transcription profiling of the Arctic springtail Megaphorura arctica after cold or salt dehydration to study insect cyroprotective dehydration

Project description:The 13,824 cDNA microarray was constructed by printing 6912 PCR-amplified cDNA clones in duplicate. These were derived from a previous EST study (Clark et al, 2007) and comprised 3840 clones from the Library D2 (fully dehydrated animals, cooled to -14°C) and 3072 clones from the Library D1 (dehydrated animals cooled to -2°C). The Stratagene SpotReport Alien Array Validation System (Stratagene, La Jolla, CA, USA) was included on the microarray. Construction and hybridization of the arrays were performed as described in Purac et al (2008) with the exception of the amino-modified primers used for the initial cDNA amplification for array printing, which in this study were: (pAL32FOR: TTCTCGGGAAGCGCG and M13 forward: GTAAAACGACGGCCAG). Hybridizations were performed using control animals in combination with the groups listed below. Six groups of animals were used for the hybridizations. Treatments were as follows: C = control = live animals from +5°C M2 = cold dehydrated animals, cooled to -2°C at a rate of 2°C/week M7 = cold dehydrated animals, cooled to -7°C at a rate of 2°C/week H18 = animals taken from -7°C, and left to recover for 18 hours at 4°C with moisture. Salt 0.9 = animals slowly dehydrated over a saturated solution of sodium nitrate (which gives a constant humidity of 98% RH) to produce animals with a similar water content as the M2 animals Salt 0.2 = as for Salt 0.9, but the animals were dehydrated to a similar water content to the M7 animals. 6 biological replicates were performed for each condition, as well as some dye swaps.

2009-07-21 | E-MEXP-2105 | biostudies-arrayexpress

Transcription profiling of Laternula elliptica under heat stress

Project description:Microarray analysis of gene expression in the Antarctic clam Laternula elliptica after exposure to elevated temperatures

2010-08-03 | E-MEXP-2336 | biostudies-arrayexpress

Transcription profiling of the Antarctic springtail Cryptopygus antarcticus kept at -15 to 2 degrees C to investigate cold tolerance

Project description:The ability of the Antarctic microarthropod, Cryptopygus antarcticus (Collembola, Isotomidae), to survive low temperatures has been well studied at the physiological level. These investigations have indicated the importance of the moulting process in conferring this ability. This study investigated gene expression in groups of C. antarcticus that have distinct differences in their ability to survive low temperatures. A microarray containing 5,400 C. antarcticus expressed sequence tags was used to investigate gene expression differences between groups of animals with different supercooling points (SCP), and to low temperatures close to their SCP. By demonstrating the involvement of moulting genes in the differential survival of two groups of C. antarcticus with distinct SCPs, the results of this investigation add support to the role moulting plays in conferring cold tolerance in C. antarcticus.

2009-12-01 | E-MEXP-2262 | biostudies-arrayexpress

Transcription profiling of 13.5dpc embryonic gonad/mesonephros from male Mrotm1H/Mrotm1H mice vs male wild type littermates

Project description:Direct comparison of gene expression in 13.5dpc embryonic gonad/mesonephros between male Mrotm1H/Mrotm1H mice and male wild-type littermates. Two independent pools of 10 gonads (5 mice) were compared on 4 microarrays (two colourswaps).

2007-01-09 | E-MEXP-527 | biostudies-arrayexpress