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Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with either Arabidopsis LFY or Leanworthia crassa LFY to investigate evolutionary divergence of LFY function

ABSTRACT: The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from Leanworthia crassa (Lcr) to complement an Arabidopsis LFY mutant. Plants used are homozygous lfy6 mutants (EMS alleles) in Ler background which are transformed or not (for the lfy6 mutant) by genomic clones for Arabidopsis LFY (AthLFY) or Leanworthia crassa LFY (LcrLFY). Flowering was synchronized by growing plants in SD then shifting them to LD. 2 time points samples (wild type Ler) were taken at the end of the SD period as a reference for genes induced by shifting to LD, irrespective of the status at the LFY locus.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: D. Baum

PROVIDER: E-MEXP-682 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Evolutionary divergence of LFY function in the mustards Arabidopsis thaliana and Leavenworthia crassa.

Sliwinski M K MK White M A MA Maizel A A Weigel D D Baum D A DA

Plant molecular biology 20060817 1-2

LEAFY (LFY), a transcription factor involved in the regulation of flower development in Arabidopsis thaliana, has been identified as a candidate gene in the diversification of plant architecture in Brassicaceae. Previous research with Leavenworthia crassa, which produces solitary flowers in the axils of rosette leaves, has shown that the L. crassa LFY ortholog, LcrLFY, rescues most aspects of flower development in A. thaliana but showed two novel traits: flowers produced additional petals and in ...[more]

PMID: 16915521

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Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with different LFY orthologs to investigate evolutionary divergence of LFY function

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2009-07-23 | E-MEXP-2258 | biostudies-arrayexpress

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