Project description:Response of C. elegans to Serratia marcesens infection at 48 hours.This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.
Project description:Response of C. elegans to Serratia marcesens infection at 24 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter
Project description:Response of C. elegans to infection by Drechmeria coniospora at 12 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.
Project description:Response of C. elegans to Drechmeria coniospora infection at 24 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.
Project description:Transcritome study of C.elegans exposed to multiple, different bacterial pathogens. Experiments were performed in set-replicates of either 3 or 5.<br> There are 3 for samples: Aeromonas hydrophila, Enterococcus faecalis, Erwinia carotovora and Photorhabdus luminescens. <br> There are 5 for samples: Serratia marcesens and Escherichia coli (control).<br>
Project description:1.Sample Growth Conditions protocols<br> Eggs from IG274 wt; frIs7 and dapk-1(ju4); frIs7 worms cultivated at 25°C were allowed to hatch in the absence of food at 25°C for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours.<br> 2.Sample Treatment protocols <br> Synchronised IG274 wt; frIs7 and CZ5701 dapk-1(ju4); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.