Project description:1.Sample Growth Conditions protocols<br> Eggs from IG274 wt; frIs7 and IG339 nipi-1(fr1); frIs7 worms cultivated at 25ðC were allowed to hatch in the absence of food at 25ðC for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours.<br> 2.Sample Treatment protocols <br> Synchronised IG274 wt; frIs7 and IG339 nipi-1(fr1); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.
Project description:1.Sample Growth Conditions protocols<br> Eggs from IG274 wt; frIs7 and dapk-1(ju4); frIs7 worms cultivated at 25°C were allowed to hatch in the absence of food at 25°C for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours.<br> 2.Sample Treatment protocols <br> Synchronised IG274 wt; frIs7 and CZ5701 dapk-1(ju4); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.
Project description:Transcritome study of C.elegans exposed to multiple, different bacterial pathogens. Experiments were performed in set-replicates of either 3 or 5.<br> There are 3 for samples: Aeromonas hydrophila, Enterococcus faecalis, Erwinia carotovora and Photorhabdus luminescens. <br> There are 5 for samples: Serratia marcesens and Escherichia coli (control).<br>
Project description:Transcriptional response of C.elegans against nematocidal B. thuringiensis. Experiments were performed in set-replicates of either 3 for the C. elegans strain N2 or 4 for the strains MY15 and MY18.
Project description:E. coli lipopolysacharide was treated on SL2* cells for 1hr, 4 and 12hrs respectivly. cDNA from those cells was compared with control. For one sample, cyclohexamide was treated for 1hr and then LPS was treated for 4hrs. Then, compared with control.
Project description:Genome-wide in planta determination of the quorum sensing regulon in Pectobacterium atrosepticum, through gene expression analysis of ExpI mutants using Agilent custom microarrays.
Project description:Response of C. elegans to infection by Drechmeria coniospora at 12 hours. This submission is not MIAME compliant as only a subset of each raw data file has been supplied by the submitter.