Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genotyping of twenty Pseudomonas aeruginosa strains

ABSTRACT: 20 P. aeruginosa strains were genotyped using a custom spotted oligo array (Ausubel P. aruginosa genotyping 4.8K v1), which included primarily oligos for genes present in strain PA14 but absent in PAO1, and oligos for genes present in PAO1 but absent in PA14 (as well as controls).

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Laurence Rahme

PROVIDER: E-MEXP-824 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial.

Lee Daniel G DG Urbach Jonathan M JM Wu Gang G Liberati Nicole T NT Feinbaum Rhonda L RL Miyata Sachiko S Diggins Lenard T LT He Jianxin J Saucier Maude M Déziel Eric E Friedman Lisa L Li Li L Grills George G Montgomery Kate K Kucherlapati Raju R Rahme Laurence G LG Ausubel Frederick M FM

Genome biology 20061012 10

<h4>Background</h4>Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an important opportunistic human pathogen. Generally, the acquisition of genes in the form of pathogenicity islands distinguishes pathogenic isolates from nonpathogens. We therefore sequenced a highly virulent strain of P. aeruginosa, PA14, and compared it with a previously sequenced (and less pathogenic) strain, PAO1, to identify novel virulence genes.<h4>Results</h4>The PA14 and PAO1 genomes are remarkably si ...[more]

PMID: 17038190

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Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages.<br><br>We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis.<br><br>We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation.<br><br>Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

Genotyping of twenty Pseudomonas aeruginosa strains

Publications

Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial.

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