Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human chondrosarcoma cells overexpressing SOX9

ABSTRACT: SOX9 was overexpressed in SW1353 chondrosarcoma cells. RNA extracted from these cell or form those transduced with a control retrovirus were compared by microarray analysis on "Human known gene SGC oligo set array number 1" glass spotted arrays obtained from HGMP. One RNA sample was derived from each cell type and was subjected to 4 repeated hybridisations. 2 of these were dye swaps.

INSTRUMENT(S): GenePix Personal 4100A [Axon Instruments]

ORGANISM(S): Homo sapiens

SUBMITTER: Colette Redmond

PROVIDER: E-MEXP-826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Comparative genomic hybridization of samples from patients with head and neck squamous cell carcinoma and anemia against normal DNA

Project description:Anaemia negatively affects the prognosis of patients with head and neck squamous cell carcinoma (HNSCC). This study investigates whether anaemia is associated to genetic changes and whether these changes predict the clinical outcome.

2007-12-27 | E-TABM-359 | biostudies-arrayexpress

Comparative genomic hybridization of 33 group I. Clostridium botulinum strains reveals 2 clusters with different resistance to cadmium and arsenic

Project description:genomic comparison of group I C.botulinum strains

2009-03-26 | E-TABM-309 | biostudies-arrayexpress

Comparative genomic hybridization of DNA from Lepidium sativum and Arabidopsis thaliana

Project description:An experiment was performed to compare genomic DNA from Lepidium sativum and Arabidopsis thaliana, on microarrays spotted with CATMA Arabidopsis GSTs

2010-10-02 | E-TABM-744 | biostudies-arrayexpress

Ribonucleoprotein immunoprecipitation of myosin proteins and myosin-specific chaperones in fission yeast

Project description:Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using various myosin proteins and myosin-specific chaperones from the fission Schizosaccaromyces pombe

2010-03-01 | E-TABM-335 | biostudies-arrayexpress

Ribonucleoprotein immunoprecipitation of S. pombe cells during meiosis using TAP-tagged RNA-binding protein Meu5

Project description:Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using the RNA-binding protein Meu5 from the fission Schizosaccaromyces pombe

2011-01-01 | E-TABM-777 | biostudies-arrayexpress

Transcription profiling of mouse aorta (whole or regions) from mice of different embryonic ages

Project description:In order to identify novel regulators of haematopoietic stem cell emergence in the mouse embryonic AGM region, 3 different types of gene expression comparisons were performed. In the first one, whole dorsal aortas from embryonic day (E) 11 embryos and E9 embryos were dissected, tissues from each time point were pooled and their expression profile compared (whole aorta (WA) E9 vs E11). 3 different pools/biological replicas were obtained for each time point, a dye swap was included in each experiment, a technical replicate was performed for each pool (including the dye swap) and 2 self-self hybridisation controls were included. In the second comparison, GFP+ cells were isolated by fluorescence-activated cell sorting from pooled E9 and E11 aortas from transgenic embryos that express GFP under the regulatory elements of the Ly-6A gene. We obtained one pool for each time point and compared their transcription profile (GFP E9 vs E11). This comparison also contained a dye swap and a technical replicate (also of the dye swap). In the third comparison, the E11 aorta was cut into 3 roughly equal parts. Pools were obtained of the middle region ("m") and the two outer regions, rostral and caudal, together ("r+c") and their expression profile compared (11AO m vs r+c). There was one pool of each population, and the experiment included a dye swap and a technical replicate (also of the dye swap) and one self-self hybridisation control. Isolated RNA was amplified by T7-mediated in vitro transcription, with one round for the first and the third comparison and two rounds for the second comparison (sorted GFP+ cells). Probes were labelled during the reverse transcription step. Within array normalisation was achieved with Lowess Smooth and between arrays with Z scores. ANOVA analysis identified differentially expressed genes and K means clustering was used to segregate them into upregulated and downregulated genes.

2009-06-06 | E-TABM-659 | biostudies-arrayexpress

Ribonucleoprotein immunoprecipitation of fission yeast to study myosin proteins and other cytoskeletal components

Project description:Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using various myosin proteins and other cytoskeletal components from the fission Schizosaccaromyces pombe

2009-09-01 | E-TABM-656 | biostudies-arrayexpress

Ribonucleoprotein immunoprecipitation of fission yeast to study myosin proteins and myosin-specific chaperones

Project description:Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using various myosin proteins and myosin-specific chaperones from the fission Schizosaccaromyces pombe. Two strains were used. In rng3TAP the rng3 protein has been tagged with TAP to allow its detection and immunoprecipitation. The TAP strain expresses the TAP sequence alone (without being attached to an other protein)

2008-12-23 | E-TABM-516 | biostudies-arrayexpress

Transcription profiling of 23 human thyroid tissues including 11 normal tissue, 4 follicular thyroid adenomas and 8 follicular thyroid carcinomas, in order to identify molecular profile associated with PAX8PPARgamma translocation

Project description:Study of a series of 23 human thyroid tissues including 11 normal tissue, 4 follicular thyroid adenomas and 8 follicular thyroid carcinomas, in order to identify molecular profile associated with PAX8PPARgamma translocation. The main factor is presence of PAX8PPARgamma translocation. Other clinical factors are also described for each sample. (see supplementary Tables). The reference used is a pool of equal quantities of mRNA from each of the 23 samples studied.

2005-06-28 | E-TABM-11 | biostudies-arrayexpress

Transcription profiling of brain from 1-day-old and forager Apis mellifera, Apis cerana, Apis dorsata, Apis florea bees to compare behavioral maturation

Project description: Not available

2007-05-16 | E-TABM-262 | biostudies-arrayexpress