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Transcription profiling of wild type, waaG mutant and rfaH mutant Salmonella enterica serovar Enteritidis PT4 and serovar Typhimurium to investigate live-attenuated vacine candidates

ABSTRACT: Effect of mutation of rfaH on gene expression in Salmonella enterica serovar Enteritidis PT4 and Salmonella enterica serovar Typhimurium 4/74

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Salmonella enterica

SUBMITTER: Levente Emody

PROVIDER: E-MEXP-844 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Down-regulation of key virulence factors makes the Salmonella enterica serovar Typhimurium rfaH mutant a promising live-attenuated vaccine candidate.

Nagy Gábor G Danino Vittoria V Dobrindt Ulrich U Pallen Mark M Chaudhuri Roy R Emödy Levente L Hinton Jay C JC Hacker Jörg J

Infection and immunity 20061001 10

Mutants of Salmonella enterica serovar Typhimurium that lack the transcriptional regulator RfaH are efficient as live oral vaccines against salmonellosis in mice. We show that the attenuation of the vaccine candidate strain is associated with reduced net growth in epithelial and macrophage cells. In order to identify the relevant RfaH-dependent genes, the RfaH regulon was determined with S. enterica serovars Enteritidis and Typhimurium using whole-genome Salmonella microarrays. As well as impact ...[more]

PMID: 16988271

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