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Transcription profiling of colon mucosa from rats fed a high fat, high sucrose, low fibre diet with and without red wine polyphenols

ABSTRACT: In the experiment two groups of rats were compared. The control group consisted of 10 male, 5- to 6-weeks old, Fischer 344 (F344) rats (Nossan, Correzzana, Milan, Italy) fed a high fat, high sucrose, low fibre diet (control diet) for two weeks. This diet was based on the AIN76 diet [19], and was modified to contain 23% (w/w) fat (from corn oil) and a low level of cellulose (2% w/w), to mimic the high risk of colon cancer in human populations consuming high fat diets. The experimental group consisted of 10 male, 4- to 5-weeks old, F344 rats fed the same high fat diet as the control group in combination with 50 mg/kg red wine polyphenols for two weeks. The red wine polyphenol extract was prepared as described by Femia et al.
Total RNA was extracted using the RNeasy Midi kit (Qiagen, Milan, Italy). Equally amounts of RNA extracted from the colon mucosa of control diet-fed rats (n=10) were pooled and used as common reference for all hybridizations.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Rattus norvegicus

SUBMITTER: Rachel van Haaften

PROVIDER: E-MEXP-934 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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