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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat colonic tumors induced by 1,2-dimethylhydrazine (DMH)

ABSTRACT: Gene expression analysis of 1,2-dimethylhydrazine (DMH)-induced colon cancer in F344 rats. 9 tumours and their paired normal mucosa were hybridized on 4x44K Agilent Whole rat arrays.

ORGANISM(S): Rattus norvegicus

DISEASE(S): normal

SUBMITTER: Cristina Luceri

PROVIDER: E-MEXP-2471 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profile and genomic alterations in colonic tumours induced by 1,2-dimethylhydrazine (DMH) in rats.

Femia Angelo Pietro AP Luceri Cristina C Toti Simona S Giannini Augusto A Dolara Piero P Caderni Giovanna G

BMC cancer 20100511

<h4>Background</h4>Azoxymethane (AOM) or 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats shares many phenotypical similarities with human sporadic colon cancer and is a reliable model for identifying chemopreventive agents. Genetic mutations relevant to human colon cancer have been described in this model, but comprehensive gene expression and genomic analysis have not been reported so far. Therefore, we applied genome-wide technologies to study variations in gene expression and ...[more]

PMID: 20459814

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Transcription profiling of rat colon tumors induced by DMH

Project description:344 rats were treated twice, one week apart, with subcutaneous injections of 1,2-dimethylhydrazine (150 mg/kg x 2). Thirty-two weeks after the first DMH injection, rats were sacrificed and colonic tumours (T) and normal colon mucosa (NM) harvested and stored in RNAlater. Genomic DNA was extracted from ten samples of paired T and NM and hybridized on Agilent Rat Genome CGH Microarray 2x105K.

2010-07-20 | E-MEXP-2487 | biostudies-arrayexpress

A diet enriched with biologically active substances as a protecting factor against carcinogenesis process induced by 1,2-dimethylhydrazine in rat liver

Project description:The aim of this study was to evaluate the ability of a diet enriched with biologically active compounds to protect against 1,2-dimethylhydrazine - induced carcinogenesis in 14-month old rats liver. Rats from control and experimental groups after 14 months of experiment were given 5 times 1,2-dimethylhydrazine (DMH) by intraperitoneal treatment at a dose of 30mg/kg of body weight once a week to induce the process of carcinogenesis. RNA from their livers were hybridized to Agilent two color microarrays with a common reference. Then, the transcriptomic profile of these livers were compared to the transcriptomic profile of 14-month rats received the same control diet and diet enriched with biologically active substances, but without 1,2-dimethylhydrazine - induction (data from GEO Submission - GSE51657).

2014-02-27 | E-GEOD-55378 | biostudies-arrayexpress

Gene expression profiling of colon mucosa after cytotoxic insult in wild type and Apc-mutated rats: possible relation with resistance to apoptosis during carcinogenesis

Project description:Gene expression profiles of the colon mucosa of wild type and PIRC rats after 1,2-dimethylhydrazine (DMH)-treatment

2016-11-03 | E-MTAB-4910 | biostudies-arrayexpress

Transcription profiling of rat sarcopenic skeletal muscle after treatment with extract of Ginko biloba Egb 761 shows that a juvenile gene expression profile is reactivated

Project description:The sarcopenia-associated decline of the muscular performances in older people is improved by exercise and caloric restriction but is still lacking a more suitable pharmacological treatment. We investigated the effects of EGb 761, a Gingko biloba extract used to treat chronic age-dependent neurological disorders, on the soleus muscle of aged rats. DNA microarray analysis shows that the EGb 761 treatment is associated with the transcriptional reprogramming of genes related to regeneration and energy production functions. EGb 761 up-regulated genes implicated in myogenesis though the TGF? signaling pathway. Also, EGb 761, by modulating genes implicated in fatty acids and glucose oxidation, reversed the agerelated shift from lipids to glucose and improved energy production in mitochondria. These modifications restore a more juvenile gene expression pattern that is concomitant with the improvement of the muscular performances, as assessed by electrophysiological and biochemical parameters. Thus, EGb 761 may represent a novel treatment for sarcopenia

2009-06-30 | E-MEXP-1937 | biostudies-arrayexpress

Transcription profiling of rat radiation-induced osteosarcomas

Project description:Identification of dysregulated genes involved in the radiation-induced tumorigeneis of bone.

2009-08-05 | E-MEXP-1752 | biostudies-arrayexpress

Transcription profiling of rat brain after prolonged administration of Phenytoin

Project description:In order to elucidate the molecular mechanisms of action of Phenytoin, we examined by microarrays the effects of prolonged administration of Phenytoin on gene expression in hippocampus and frontal cortex of Sprague-Dawley rats chronically treated with Phenytoin.

2009-12-31 | E-MEXP-1728 | biostudies-arrayexpress

Transcription profiling of rat mixed-tissue RNA samples in different laboratories to monitor proficiency

Project description:A pilot program for monitoring proficiency in microarray facilities using three replicates of two different RNA sources. Participating laboratories prepared and hybridized targets from the six RNA samples using their own protocols. The entire process was repeated three times over a nine-month period and included approximately fifteen distinct laboratories in each testing round.

2008-09-12 | E-MEXP-1568 | biostudies-arrayexpress

Gene expression profile of butyrate mediated prevention of coloractal cancer in a mouse model

Project description:To further elucidate the mechanism of butyrate mediated chemoprevention of colorectal cancer,we have employed whole genome microarray expression profiling as a discovery platform to identify genes that differentially expressed in mice from Butyrate group compared those from DMH group. The mice of DMH group were received normal diet (calcium concentration,1.24%) and subcutaneous injection of 1,2-Dimethylhydrazine at a dose of 20 mg/kg once weekly for 20 weeks; the mice of Butyrate group were received high calcium diet (calcium concentration,3%) with 1.5% Butyrate and subcutaneous injection of 1,2-Dimethylhydrazine at a dose of 20 mg/kg once weekly for 20 weeks. The mice were sarcrificed at 24 week. Nine normal colonic mucosa (3 from DMH group, 3 from Butyrate group mice with tumors, and 3 from Butyrate group mice without tumors) were used for the microarray analysis. The differentially expressed genes were identified between butyrate group and DMH group,as long as differentially expressed pathways and gene ontology terms.

2011-12-31 | E-GEOD-25858 | biostudies-arrayexpress

Gene expression profile of calcium mediated prevention of colorectal cancer in a mouse model

Project description:To further elucidate the mechanism of calcium mediated chemoprevention of colorectal cancer,we have employed whole genome microarray expression profiling as a discovery platform to identify genes that differentially expressed in mice from high calcium group compared those from DMH group.The mice of DMH group were received normal diet (calcium concentration,1.24%) and subcutaneous injection of 1,2-Dimethylhydrazine at a dose of 20 mg/kg once weekly for 20 weeks,the mice of high calcium group were received high calcium diet(calcium concentration,3%) and subcutaneous injection of 1,2-Dimethylhydrazine at a dose of 20 mg/kg once weekly for 20 weeks.The mice were sarcrificed at 24 week.Nine normal colonic mucosa (3 from DMH group, 3 from high calcium group mice with tumors, and 3 from high calcium group mice without tumors) were used for the microarray analysis. The differentially expressed genes were indentified between high calcium group and DMH group,as long as differentially expressed pathways and gene ontology terms.

2011-11-23 | E-GEOD-25680 | biostudies-arrayexpress

MicroRNA profiling of human AGS gastric cancer cell line was treated with the selective COX-2 inhibitor celecoxib

Project description:AGS gastric cancer cell line was treated with the selective COX-2 inhibitor celecoxib. microRNA expression profile was analyzed between untreated cells and cells treated with celecoxib.

2009-07-11 | E-MEXP-2231 | biostudies-arrayexpress

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