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Transcription profiling of Pseudomonas putida in the rhizosphere of corn

ABSTRACT: It has been performed a genome-wide analysis of gene expression of the root-colonizing bacterium Pseudomonas putida KT2440 in the rhizosphere of corn (Zea mays var. Girona. To identify reliable rhizosphere differentially expressed genes, rhizosphere populations of P. putida bacteria cells were compared with three alternative controls: i) planktonic cells growing exponentially in rich medium (LB), ii) planktonic cells in stationary phase in LB, and iii) sessile populations established in sand microcosms, under the same conditions used to grow inoculated corn plants.

INSTRUMENT(S): GenePix Personal 4100A [Axon Instruments]

ORGANISM(S): Pseudomonas putida

SUBMITTER: Maria Isabel Ramos-Gonzalez

PROVIDER: E-MEXP-949 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic analysis reveals the major driving forces of bacterial life in the rhizosphere.

Matilla Miguel A MA Espinosa-Urgel Manuel M Rodríguez-Herva José J JJ Ramos Juan L JL Ramos-González María Isabel MI

Genome biology 20070101 9

<h4>Background</h4>Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere).<h4>Results</h4>A global analysis of Pseudomonas putida genes expressed during their interaction with maize roots revealed how a bacterial population adjusts its genetic program to this lifestyle. Differentially expressed genes were identified by comparing rhizosp ...[more]

PMID: 17784941

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