Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of mouse naive CD8+ T-cell differentiation during a primary immune response

ABSTRACT: Title: Gene Expression analysis of naive CD8+ T-cell differentiation during a primary immune response Description: C57BL/6 wild type mice are administered GK1.5 (depleting anti CD4 antibody) or isotope control by intraperitoneal injection at 3 and 1 day pre-infection, then every 36-48 hour post infection. 24 hours pre-infection, splenocytes from F5 RAG -/- mice are stained with CFSE and 10^7 splenocytes are transfered to recipient C57BL/6 mice by intravenous injection. 24 hours laters, recipient mice are infected with 10^7 PFU of a recombinant Vaccinia virus expressing NP366-74. At timepoints 0, 12, 24, 48, 72 and 96 splenocytes are obtained and FACS sorted to obtain CD8+ T-cells.

ORGANISM(S): Mus musculus

SUBMITTER: unknown unknown

PROVIDER: E-MIMR-38 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human CD34+ stem/progenitor cell populations mobilized into the blood by granulocyte colony-stimulating factor

Project description:Mononuclear cells were isolated from granulocyte colony stimulating factor mobilised peripheral blood. CD34+ cells were selected and separated into adherent and non-adherent cells. Adherent and non-adherent samples were amplified and labelled using two IVT cycles, and gene expression profiling was performed by hybridisation to Affymetrix Human Genome U133 Plus2.0 Arrays.

2008-08-01 | E-MIMR-861 | biostudies-arrayexpress

Transcription profiling of human CD4CD45RO T-cells stimulated with interleukin-2, interleukin-6 or TNF-alpha and harvested at 24, 72 or 144 hours or stimulated with anti-CD3 antibodies and harvested at 24 hours

Project description:Blood taken from a healthy human donor was used to isolate CD4CD45RO T-cells. Purified cells were stimulated with either interleukin-2 (IL-2), Interleukin-6 (IL-6) and TNF-alpha and harvested at 24 hours, 72 hours (3 days) and 144 hours (6 days) or anti-CD3 antibodies and harvested at 24 hours. These were compared to unstimulated purified CD4CD45RO T-cells harvested at 0 hours.

2008-06-01 | E-MIMR-341 | biostudies-arrayexpress

Transcription profiling of human chondrocytes from patients with osteosarcoma or osteomyelitis, cultured in either 1% or 20% oxygen for one or four days

Project description:Cartilage was taken from the femoral condyle and tibial plateau from eight patients, cut into small pieces and digested overnight with Collagenase Type 2. The resultant chondrocytes were put through a cell strainer, pelleted and resuspended in fresh media and cultured for one week in 20% oxygen. The culture was split into five sub-cultures, one of which was immediately frozen; the remainder were allowed to recover overnight. Cells were subsequently grown at either 1% oxygen (hypoxia simulation) or 20% oxygen (normoxia simulation) for either one or four days before RNA extraction. Labelled RNA was then hybridised to Affymetrix Human U133 plus 2.0 arrays.

2008-04-01 | E-MIMR-644 | biostudies-arrayexpress

Transcription profiling of peritoneal fat of SHR/01a (spontaneously hypertensive rat), BN-Lx (Brown Norway congenic carrying polydactyly-luxate syndrome)and recombinant inbred (HXB/BXH) 6-week old rat strains

Project description:Gene expression profiling of peritoneal fat of SHR/01a (spontaneously hypertensive rat), BN-Lx (Brown Norway congenic carrying polydactyly-luxate syndrome)and recombinant inbred (HXB/BXH) 6-week old rat strains.

2010-11-18 | E-AFMX-7 | biostudies-arrayexpress

Transcription profiling of kidney from rats of SHR/Ola, BN and SHR-18 strains after being provided with drinking water with 1% or 0% sodium chloride

Project description:Four male SHR/Ola, BN and SHR-18 rats were fed a normal diet and sacrificed at 9 weeks of age. Four male SHR/Ola and SHR-18 rats at 8 weeks of age were fed 1% NaCl for one week and then sacrificed. Kidneys were removed and frozen in liquid nitrogen for all 20 animals. Total RNA was isolated, labelled cRNA was generated and hybridised to Affymetrix Rat RG-U34ABC arrays.

2008-04-01 | E-MIMR-1122 | biostudies-arrayexpress

Transcription profiling of mouse embryonic stem cells induced to express the HOXB1 transcription factor under doxycycline tet-on control

Project description:Undifferentiated mouse embryonic stem cells stably expressing the HOXB1 transcription factor under doxycycline tet-on control were differentiated into embryoid bodies. Nestin+ neuroepithelial cells were selected by transfer of the embryoid bodies to ISTFn media. Expression of HOXB1 was induced by addition of doxycycline or not induced on the seventh day of selection and also for one day after trypsination and transfer to laminin/polyornithine substrate on matrigel substrate.

2008-06-30 | E-MIMR-441 | biostudies-arrayexpress

Transcription profiling of BXH/HXB rat recombinant inbred strains derived from the spontaneously hypertensive rat (SHR/Ola) and the Brown Norway congenic strains carrying the polydactylyl-luxate mutation (BN-Lx)

Project description:BXH/HXB rat recombinant inbred (RI) strains are derived from the spontaneously hypertensive rat (SHR/Ola) and the Brown Norway congenic strains carrying the polydactylyl-luxate mutation (BN-Lx). Tissue from the apex of left ventricle of the heart was disected from 128 RI SHR/Ola and BN-Lx parental strains, RNA was extracted and labelled and hybridised to Affymetrix Rat Genome 230 2.0 Arrays.

2008-04-01 | E-MIMR-222 | biostudies-arrayexpress

Transcription profiling of ovarian gene expression in RIP140 wild type, heterozygous and knock out mice

Project description:Title: Comparison of ovarian gene expression in RIP140 wild type, heterozygous and knock out mice. Description: RIP140 null mice fail to ovulate. This experiment was designed to determine the gene expression profile of these animals compared to wild type and heterozygous animals following hormone treatments that induce the biological changes required for ovulation to occur.

2005-05-11 | E-MIMR-43 | biostudies-arrayexpress

Transcription profiling of PPAR alpha function in fibrate fed animals during light and dark cycles

Project description:Title: Study of PPAR alpha function in fibrate fed animals during light and dark cycles. Description: Study of PPAR alpha function in fibrate fed animals during light and dark cycles.

2005-05-10 | E-MIMR-2 | biostudies-arrayexpress

Transcription profiling of rat primary cardiac myocytes treated with endothelin-1, cycloheximide, azekenpaullone or combinations of these before extraction of total or polysomal RNA

Project description:Ventricles were removed from 2-day Sprague_Dawley rat litters from which primary cardiac myocytes (>95%) were isolated. Myocytes were either untreated or exposed to either endothelin-1 for 30, 60 or 120 minutes, cycloheximide for 70 or 130 minutes, endothelin-1 for 10 minutes followed by cycloheximide for 70 minutes, azekenpaullone for 60 minutes before extraction of total or polysomal (actively transcribed) RNA. Labelled cRNA was hybridised to Affymetrix Rat 230 2.0 arrays.

2008-02-17 | E-MIMR-681 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data