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Gene expression analysis in KG1a cells overexpressing either NO66wt or NO66mut

ABSTRACT: The AML cell line KG1a does not express the nucleolar protein NO66. We generated stable cell lines overexpressing NO66wt and NO66mut, respectively, in order to look for differential gene expression compared to the original cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Marion Schmidt-Zachmann

PROVIDER: E-MTAB-10064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The JmjC-domain protein NO66/RIOX-1 affects the balance between proliferation and maturation in acute myeloid leukemia.

Yu Weijia W Lutz Christoph C Krämer Alwin A Schmidt-Zachmann Marion S MS

Experimental cell research 20210318 1

As epigenetic regulators are frequently dysregulated in acute myeloid leukemia (AML) we determined expression levels of the JmjC-protein NO66 in AML cell lines and sub fractions of healthy human hematopoietic cells. NO66 is absent in the AML cell lines KG1/KG1a which consist of cells with the immature CD34+/CD38- phenotype and is regarded as a "stem cell-like" model system. Similarly, NO66 is not detectable in CD34+/CD38- cells purified from healthy do ...[more]

PMID: 33745927

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