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ChIP-Seq to determine binding sites of transcription factor BldO in the genome of Streptomyces venezuelae

ABSTRACT: The transcription factor BldO was identified as a target of WhiAB in an earlier ChIP-Seq experiment. This experiment was conducted to determine the targets of BldO in the Streptomyces venezuelae genome.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Streptomyces venezuelae

SUBMITTER: Mark Buttner

PROVIDER: E-MTAB-10250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Multi-layered inhibition of Streptomyces development: BldO is a dedicated repressor of whiB.

Bush Matthew J MJ Chandra Govind G Findlay Kim C KC Buttner Mark J MJ

Molecular microbiology 20170327 5

BldD-(c-di-GMP) sits on top of the regulatory network that controls differentiation in Streptomyces, repressing a large regulon of developmental genes when the bacteria are growing vegetatively. In this way, BldD functions as an inhibitor that blocks the initiation of sporulation. Here, we report the identification and characterisation of BldO, an additional developmental repressor that acts to sustain vegetative growth and prevent entry into sporulation. However, unlike the pleiotropic regulato ...[more]

PMID: 28271577

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