Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

33 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of KYAE-1 and OE19 oesophageal adenocarcinoma cells following induction of dominant negative (acidic) FOS

ABSTRACT: To identify AP-1 regulated genes in KYAE-1 and OE19 oesophageal adenocarcinoma cells we induced expression of dominant negative (acidic) FOS to inhibit AP-1 activity and then performed RNA-seq. Acidic FOS (aFOS, J Biol Chem. 1997 Jul 25;272(30):18586-94. doi: 10.1074/jbc.272.30.18586) was expressed from the pINDUCER20 vector.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER:

PROVIDER: E-MTAB-10334 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP128193

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of KYAE-1 and OE19 oesophageal adenocarcinoma cells following induction of dominant negative (acidic) FOS

Project description:RNA-seq of KYAE-1 and OE19 oesophageal adenocarcinoma cells following induction of dominant negative (acidic) FOS

| PRJEB44174 | ENA

H2AZ extended acidic patch is necessary for formation specialized chromatin states in ESCs [RNA-Seq]

Project description:The H2A variant H2AZ is essential for embryonic development and for proper execution of developmental gene expression programs in embryonic stem cells (ESCs). Divergent regions in H2AZ are likely key for its functional specialization, but we know little about how these differences contribute to chromatin regulation. Here, we show that the extended acidic patch, specifically the three divergent residues in the C-terminal docking domain, is necessary for lineage commitment during ESC differentiation and proper execution of gene expression programs during ESC differentiation. Surprisingly, disruption of the acidic patch domain has a distinct consequence on cellular specification compared to H2AZ depletion. This is consistent with differences in gene expression profiles of H2AZ M-bM-^@M-^Sdepleted and acidic patch (AP) mutant ESCs during early lineage commitment. Interestingly, the distinct consequence of AP mutant expression on gene regulation is coincidence with an altered destabilized chromatin state and high chromatin mobility dependent on active transcription. Collectively, our data shows that the divergent residues within the acidic patch domain are key structural determinants of H2AZ function and links chromatin structure and dynamics with gene regulation and cell fate specification. H2AZ extended acidic patch was mutated, or H2AZ was KD in mouse embryonic stem cells and RNA-Seq analysis was performed on the resulting cultures. Characterization of H2AZ-WT and -AP3-mutant binding specificities were performed by ChIP-Seq.

2013-09-23 | E-GEOD-40064 | biostudies-arrayexpress

H2AZ extended acidic patch is necessary for formation specialized chromatin states in ESCs [ChIP-Seq]

2013-09-23 | E-GEOD-40063 | biostudies-arrayexpress

Genome-wide mapping of Fos binding site (K562)

Project description:Proto-oncogene c-Fos is a leucine-zipper containing transcription factor which has a DNA binding domain and a transactivation domain. Fos dimerizes with another transcription factor Jun to form AP1 transcription factor. The AP-1 complex has been implicated in transformation and progression of cancer. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Map binding sites of transcription factor Fos in the genome of K562 cells. The K562 input data has been deposited in GEO as GSM325934.

2009-12-31 | E-GEOD-19546 | biostudies-arrayexpress

KAS-seq of OE19 cell line

Project description:To investigate changes to eRNA transcripts in the presence and the absence of lapatinib in OE19 cells, we performed KAS-seq on OE19 cells treat with 500 nM lapatinib for 1 day.

2024-09-25 | E-MTAB-14095 | biostudies-arrayexpress

KAS-seq of OE19 cells

Project description:To identify eRNA transcripts in OE19 cells, we performed KAS-seq.

2023-02-24 | E-MTAB-11357 | biostudies-arrayexpress

Investigating the role of FOS/AP-1 in OnF reprogramming during tumor intiation

Project description:To investigate the role of FOS/AP-1 in early OnF reprogramming, we blocked its activity during tumor intiation by overexpressing a dominant negative form of FOS.

2024-10-17 | GSE263056 | GEO

KAS-seq of OE19 cell line

Project description:To investigate changes to eRNA transcripts in OE19 cells

2024-09-25 | E-MTAB-14098 | biostudies-arrayexpress

The function of c-Fos in hepatocarcinogenesis

Project description:c-Fos, a member of the stress-activated Activator Protein 1 (AP-1) transcription factor family, is expressed in human hepatocellular cancer (HCC). Using genetically engineered mouse models (GEMMs) we show that hepatocyte-specific expression of c-Fos leads to a proliferative, de-differentiated phenotype, whereas hepatocyte-specific deletion of c-Fos protects against diethylnitrosamine (DEN)-induced liver cancer. Furthermore, c-Fos-expressing livers resemble human HCCs based on expression profiles. In the present RNA seq, we intend to analyze the transcriptomic profile of livers at 2 and 4 mo hepatocyte-specific c-Fos expression compared to the corresponding age-matched control mice. Moreover, we analyzed livers of mice with hepatocyte-specific deletion c-Fos at 48h after DEN treatment compared to identically treated control mice.

2017-03-23 | GSE81079 | GEO

RNA-seq of the OE19 cell line

Project description:Sequencing the nuclear RNA in OE19 cells by RNA-seq

2024-09-25 | E-MTAB-14093 | biostudies-arrayexpress