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RNAseq of Huh7 and Huh7.5 cells treated with interferon alpha, beta or lambda.

ABSTRACT: The transcriptome of Huh7 and Huh7.5 was sequenced from cells treated with interferon alpha (IFNα), beta (IFNβ) or lambda (IFNλ) for 8 hours. Each treatment was compared to respective mock-treated cells in 6 pairwise comparisons, for a total of 43 samples classified in 12 experimental conditions. Cells were treated for 8h with 10ng/ml of Interferon-α2A, 5000 U/ml of Interferon-β1A or 100ng/ml of interferon-λ3 prior RNA extraction and library preparation. Mock-treated cells were used as control for each treatment. 3 to 4 biological replicates were analysed for each biological condition. Libraries for Illumina sequencing were constructed by polyA selection with the Illumina Kit. A paired end (2x100) run was performed.

INSTRUMENT(S): Illumina HiScanSQ

ORGANISM(S): Homo sapiens

SUBMITTER: Riccardo Rossi

PROVIDER: E-MTAB-10469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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