Cross-platform standardization for the identification of circulating miRNAs associated with a risk of distant recurrence in oestrogen receptor-positive breast cancers.

ABSTRACT: Circulating microRNAs (miRNAs) may be able to serve as prognostic biomarkers. However, identifying the accurate screening method/s for miRNA detection/quantification from serum is crucial for any further downstream analysis. Here, we compared two high-throughput methods, small RNA sequencing (sRNA-seq) and TaqMan Low-Density Array (TLDA), to quantify miRNAs at baseline (time of primary surgery) in the serum from ER+ BC patients for whom the long-term clinical follow-up information (10 years) and subsequent metastasis status are known. For this study, 48 serum samples were selected based on the outcome of breast cancer patients, and therefore sub-divided in: patients with no recurrence within a 10-years period (Cohort 1, n=16), patients with early recurrence within 5 years (Cohort 2, <5

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens??

SUBMITTER: Philippe Clézardin 

PROVIDER: E-MTAB-10555 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress