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Whole culture RNA-sequencing of transient DUX4-expressed human embryonic stem cells

ABSTRACT: Doxycycline-induced and control DUX4-TetOn human embryonic stem cells (hESCs) were collected immediately, 24 h, and 48 h after 15 min, 30 min of doxycycline, or without doxycycline treatment. Moreover, doxycycline-induced hESCs were sorted by FACS with an antibody against NaPi2b (SLC34A2) at 6 h after induction and collected after a further 6 h of culture. These cells were compared with unsorted cells, cells without doxycycline treatment, and H9 primed/naive hESCs as controls.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Masahito Yoshihara

PROVIDER: E-MTAB-10569 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell RNA-sequencing of transient DUX4-expressed human embryonic stem cells

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Expression analysis in H9-hESCs to induce naive pluripotency

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2009-03-14 | E-MEXP-1741 | biostudies-arrayexpress

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2019-11-27 | PXD013067 | Pride

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Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factorl

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