Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq from tibialis muscles from rats aged 15 months (adult) and 27 months (old)


ABSTRACT: To gain new insights into molecular changes in skeletal muscle aging and disease with a special focus on differential alternative splicing and senescence, we performed RNA-seq on tibialis muscles from 15 months old (adult, a) and 27 months old (old, o) rats. Whole (w) muscle samples were prepared for both age groups (w/a and w/o). Additionally, for both age groups, muscle fibers were isolated. For adult rats, this resulted in one group (f/a). For old rats, fibers were subjected to Laser capture microdissection guided by expression of aging markers Tmem158 and Cdkn1a, giving rise to two groups, aging marker-positive fibers (f/o+) and aging marker-negative fibers (f/o-). The entire experiment comprises 5 groups (w/a, w/o, f/a, f/o+, f/o-), with 5 animals per group, ie. 25 samples total.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Rattus norvegicus

SUBMITTER: Sebastian Hoersch 

PROVIDER: E-MTAB-10582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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